Disintegration of spermatozoa : Molecular mechanism of coiled tail and round-head formation

精子的崩解：卷尾和圆头形成的分子机制

• 批准号: 17590150
• 负责人: TOYOTA Fumie
• 金额: $ 2.3万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590150/
• 关键词: Teratozoospermia; Epididymis; Sperm maturation; GOPC; GPX4; PHGP_X; SPATA19; spergen-1; Electron microscopy; Coiled tail; GOPC欠損マウス; 免疫組織化学; Spergen-1; ミトコンドリア鞘

Gopc (Golgi-associated PDZ- and coiled-coil motif-containing protein)^<-/-> mice are infertile, showing globozoospermia, coiled tails and a stratified mitochondrial sheath. Transmission electron microscope (TEM) images of the spermatozoa were studied quantitatively to analyze disorganization processes during epididymal passage. Factors maintaining straight tail and normal mitochondrial sheath were also studied by TEM and immunoflorescent microscopy.Sperm tails retained a normal appearance in the proximal caput epididymidis. Tail disorganization started between the proximal and the middle caput epididymidis, and the latter is the major site for it. The tail moved up through the defective posterior ring and coiled around the nucleus to various degrees. Tail coiling occurred in the caput epididymidis suggesting it was triggered by cytoplasmic droplet migration. SPATA19/spergen-1, a candidate mitochondrial adhesion protein, remained on the stratified mitochondria, while GPX4/ PHGPx, a major element of the mitochondrial capsule, was unevenly distributed on them. From these findings, we speculate GPX4 is necessary to maintain normal sheath structure, and SPATA19 prevents dispersal of mitochondria, resulting in a stratified mitochondrial sheath formation in Gopc^<-/-> spermatozoa. The epididymal epithelium was normal in structure and LRP8/apoER2 expression suggesting that tail abnormality is due to intrinsic sperm factors.Three cell-structures can be condisered as requisite factors for maintaining a straight tail during epididymal maturation: 1) a complete posterior ring to prevent invasion of the tail into the head compartment, 2) stable attachment of the connecting piece to the implantation fossa, 3) a normal mitochondrial sheath supported by SPATA19 and supplied with sufficient and normally distributed GPX4.

Gopc（含有高尔基体相关PDZ和卷曲螺旋基序的蛋白）^<-/->小鼠是不育的，显示出球形精子、卷曲的尾巴和分层的线粒体鞘。透射电子显微镜（TEM）图像的精子进行了定量研究，以分析在附睾通过的解体过程。透射电镜和免疫荧光显微镜观察结果表明，精子尾部在附睾近端头处保持正常形态。尾部解体起始于附睾中头和近头之间，中头是主要的解体部位，尾部通过有缺陷的后环向上移动，并不同程度地缠绕在核周围。尾部卷曲发生在附睾头，这表明它是由细胞质液滴迁移引发的。SPATA 19/spergen-1是一种候选的线粒体粘附蛋白，保留在复层的线粒体上，而GPX 4/ PHGPx是一种线粒体囊的主要成分，在它们上分布不均匀。根据这些发现，我们推测GPX 4是维持正常鞘结构所必需的，SPATA 19阻止线粒体的分散，导致Gopc^<-/->精子中分层的线粒体鞘形成。附睾上皮结构正常，LRP 8/apoER 2表达正常，提示尾部畸形是精子内在因素所致，附睾成熟过程中有三种细胞结构是维持直尾的必要因素：1）完整的后环，以防止尾部侵入头部腔室，2）连接件与植入窝的稳定连接，3）由SPATA 19支持的正常线粒体鞘，并提供充足且正常分布的GPX 4。

项目成果

セルトリ細胞・精子細胞間の特殊接合装置の機能解明の試み

试图阐明支持细胞和精子细胞之间特殊连接装置的功能

• 发表时间: 2006
• 期刊: Acta Anatomica Nipponica Vol81, Supplement
• 作者: 外山芳郎;鈴木-豊田二美枝他3名
• 通讯作者: 鈴木-豊田二美枝他3名

Factors maintaining normal sperm tail structure during epididymal maturation studied in Gopc-/-mice.

在 Gopc-/- 小鼠中研究了附睾成熟过程中维持正常精子尾部结构的因素。

• 发表时间: 2007
• 期刊: Biol. Reprod. 77
• 作者: F. Suzuki-Toyota;T. Noda;et. al. (total 8;6th)
• 通讯作者: 6th)

精子ミトコンドリア鞘の構造維持におけるSpergen-1,PHGPx apoER2の役割:GOPC欠損マウス奇形精子をモデルとして

Spergen-1、PHGPx apoER2在维持精子线粒体鞘结构中的作用：以GOPC缺陷小鼠畸形精子为模型

• 发表时间: 2006
• 期刊: Acta Anatomica Nipponica Vol81, Supplement
• 作者: 鈴木-豊田二美枝;伊藤千鶴;他5名
• 通讯作者: 他5名

Factors maintaining normal sperm tail structure during epididymal maturation studied in Gopc^<-/-> sperm

在 Gopc^</-/-> 精子中研究附睾成熟过程中维持正常精子尾部结构的因素

• 期刊: Biology of Reproduction (in press)
• 作者: Fumie Suzuki-Toyota;Chizuru Ito;Yoshiro Toyama;Mamiko Maekawa;Ryoji Yao;Tetsuo Noda;Hiroshi Iida;Kiyotaka Toshimori
• 通讯作者: Kiyotaka Toshimori

組織細胞生物学

组织细胞生物学

• 发表时间: 2006
• 作者: Kierszenbaum;AL著;内山安男監訳;年森清隆;鈴木-豊田二美枝;伊藤千鶴 他9名訳
• 通讯作者: 伊藤千鶴 他9名訳