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Localization and roles of actin and its regulation substances, microtubules, and water channels in spematogenesis.

肌动蛋白及其调节物质、微管和水通道在精子发生中的定位和作用。

基本信息

批准号：
14570003
负责人：
TOYOTA Fumie
金额：
$ 2.18万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570003/
关键词：
GOPC aquaporin 7 knockout mouse spermiogenesis sperm maturation epididymis sperm abnormality cytoskeleton Aquapotin 7 Spermatogenesis 電子顕微鏡細胞化学 GOPC knockout mouse globozoospermia Microtubule Sperm maturation Epididymis

项目摘要

Spermiogenesis is characterized by prominent morphological changes and the reduction of cell volume. It has been pointed out that actin filaments and microtubules are involved in morphogenesis, and aquaporins (AQPs), water channel molecules, are involved in volume reduction. In the present study, their involvements were analyzed by immuno-histochemistry, electron microscopy, and KO-mouse(KO-M)(1)Localization of AQP7,8,11 in germinal epithelium : It was shown that AQP7 and 11 located in the germ cells, and AQP8 located in the Sertoli cells. The result suggests that AQP7 and 11 compensate each other.(2)Analysis of spermatogenesis in AQP7-KO-M : Signet-ring like pattern representing abnormal nuclear condensation was observed in the half individuals of the KO-mice. It suggests that AQP7 is involved at least in nuclear condensation.(3)Metamorphic sperm formation in GOPC-KO-M : In GOPC(Golgi-associated PDZ-and coiled coil motif-containing protein)-KO-M, spermatozoa are infertile with round h … More ead and coiled tail. In the present study, we have investigated the process to make such morphological abnormalities. In the early spermatids, the acroplaxome, which is a sheet mainly made of actin, and bonds acrosome and the nucleus together, was formed. Acrosomal vesicles of KO-M failed to keep contact with the nucleus and to fuse each other. Then abnormal sperm formation was induced like a domino collapse. Namely, disappearance of acrosomal vesicle→deficiency of the perinuclear ring, which is the marginal structure of the acroplaxome→defective formation of the manchette made of microtubules→deficiency of the posterior ring and the presence of perinuciear cytoplasm. These results suggest that the actin and microtubules are involved in the morphogenesis in spermiogenesis. On the other hand, flagella were made normally in the testis. However, the flagella rolled up around the nucleus during the passage through the middle caput epididymidis, where the cytoplasmic drolets migrate from the neck region to the distal part of the mid-piece. It suggest that the tail coiling occur in relation with the migration of the cytoplasmic droplet. Less

精子发生的特征是显著的形态变化和细胞体积的减少。已经指出，肌动蛋白丝和微管参与形态发生，水通道蛋白（AQP），水通道分子，参与体积减小。本研究采用免疫组化、电镜和KO-小鼠（KO-M）等方法分析了AQP 7、8、11在生殖上皮中的分布：AQP 7、11定位于生殖细胞，AQP 8定位于支持细胞。结果表明，AQP 7和11相互补偿。（2）AQP 7-KO-M小鼠精子发生的分析：KO小鼠半数个体出现印戒样的细胞核异常凝聚。这表明AQP 7至少参与了核凝聚。（3）GOPC-KO-M中精子的变态形成：GOPC（Golgi-associated PDZ-and coiled coil motif-containing protein）-KO-M中精子不能生育，具有圆形的卵母细胞，精子的形态发生了改变。 ...更多信息头和盘尾。在本研究中，我们已经调查的过程中，使这种形态异常。在早期精子细胞中，形成了以肌动蛋白为主要成分的连接顶体和细胞核的顶体片。KO-M的顶体囊泡不能与细胞核保持接触，也不能相互融合。然后，异常精子形成被诱导，就像多米诺骨牌倒塌一样。即顶体囊泡消失→核周环（顶体的边缘结构）缺乏→由微管构成的柄状结构形成缺陷→后环缺乏和核周细胞质的存在。这些结果提示肌动蛋白和微管参与精子发生的形态发生。精巢内鞭毛正常。然而，鞭毛卷起的细胞核周围的通道通过中间附睾头，在那里的细胞质液滴从颈部迁移到中间片的远端部分。这表明尾卷的发生与胞质液滴的迁移有关。少