Research on the double innervation of trapezius muscle and the mechanisim of the axons extension direction

斜方肌双重神经支配及轴突延伸方向机制研究

• 批准号: 17590171
• 负责人: YAN Jun
• 金额: $ 2.3万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590171/
• 关键词: accessory nucleus; trapezius muscle; double innervation; γ motoneuron; α motoneuron; spindle; motor end plate; fluorescent dye labeling; 神経の筋内分布; γ-運動線維; 順行性標識; DiI; Biotin Dextra

The communicating branch between the ventral rami of cervical nerves and the spinal accessory nerve (SAN) has been reported to also send motor fibers to supply the trapezius. However, the motor fiber type of the communicating branch and its peripheral distribution, and the localization of motoneurons, which extend axons either directly through the SAN or through the ventral rami of cervical nerves to innervate the trapezius are still unclear. To determine the fiber elements within the communicating branch and its peripheral distribution of the motor fibers in the muscle the anterograde tracing method was used. Moreover, the localization of motoneurons, which extend axons either directly through the SAN or through the ventral rami of cervical nerves to innervate the muscle are also investigated using fluorescent dye labeling and 3D reconstruction method.The results show that a few a motor end plates from the communicating branch were observed on the extrafusal fibers, while in the muscl … More e spindle the motor elements from the communicating branch were distributed to the polar portions of the intrafusal fibers. The results indicated that the motor fibers passing through the communicating branch to supply the trapezius are mainly y motor fibers, with some a motor fibers. Moreover, the a and γ motor fibers from the communicating branch were observed in the clavotrapezius, acromiotrapezius and the rostral part of spinotrapezius. On the other hand, the motoneurons whose axons pass through the ventral rami of cervical nerves and then enter the SAN, and those extending axons directly through the SAN are distributed within the same area in anterior horn. The neurons that extend axons through the SAN had a greater diameter than those axons that pass through the cervical nerves en route to the trapezius muscle. In addition, the axons that ultimately extend through the SAN exit the spinal cord dorsolaterally, while those that pass through the cervical nerves extend out the spinal cord through the ventral roots. We consider that the neurons that extend axons through the SAN are mainly α-motoneurons and that those projecting axons through the cervical nerves to the trapezius are mainly γ-motoneurons, because the distribution and fiber types of peripheral part are agreement with the central part. Taken together, these results could explain why patients in whom the SAN was used to treat brachial plexus injury retain some control of the trapezius muscle Less

据报道，颈神经和脊柱神经（SAN）之间的腹侧拉米之间的通信分支也可以使运动纤维供应斜方肌。但是，通信分支的运动纤维类型及其周围分布以及运动神经元的定位，轴突直接通过SAN延伸，或者穿过颈神经的腹侧拉米，以支配斜方肌支配斜方肌。为了确定通信分支中的纤维元素及其在肌肉中运动纤维的外围分布，使用了顺行示踪方法。此外，还使用荧光染料标签和3D重建方法研究了运动神经元的定位，该运动神经元直接通过SAN延伸轴突，或者通过颈神经的腹侧拉米来支配肌肉，以支配肌肉。结果表明，几个电动机的电动机底板在脉冲上观察到了脉冲上的分支，同时又分配了更多的动力，而在肌肉上分布了更多的动力……恒星纤维的部分。结果表明，穿过通信分支以提供斜方肌的电动机纤维主要是Y运动纤维，并带有一些运动纤维。此外，在Clavotrapezius，Acromiotrapezius和Spinotrapezius的延髓部分中观察到了来自通信分支的A和γ运动纤维。另一方面，轴突穿过颈神经的通风拉米，然后进入SAN的运动神经元，然后进入SAN，直接通过SAN延伸的轴突在前喇叭中分布在同一区域内。延伸轴突穿过SAN的神经元的直径比那些穿过宫颈神经的轴突更大。此外，最终通过SAN延伸的轴突向后伸出脊髓，而穿过颈神经的轴突则通过腹根延伸了脊髓。我们认为，通过SAN延伸轴突的神经元主要是α-神经元，并且那些通过颈神经向斜方肌投射的轴突主要是γ-核神经元，因为外围部分的分布和纤维类型是与中心部分一致的。共同处理，这些结果可以解释为什么使用SAN的患者用于治疗臂丛神经损伤的患者保留了对斜方肌肌肉的某种控制

项目成果

Analysis of motor fibers in the communicating branch between the cervical nerves and the spinal accessory nerve to innervate trapezius in the rat

大鼠颈神经与脊副神经交通支支配斜方肌的运动纤维分析

• 发表时间: 2006
• 期刊: Okajimas Folia Anatomica Japonica 83(3)
• 作者: Jun YAN;Jiro HITOMI
• 通讯作者: Jiro HITOMI

Localization of motoneurons that extend axons through the ventral rami of cervical nerves to innervate the trapezius muscle : A study using fluorescent dyes and 3D reconstruction method

通过颈神经腹支延伸轴突以支配斜方肌的运动神经元的定位：使用荧光染料和 3D 重建方法的研究

• 发表时间: 2007
• 期刊: Clinical Anatomy 20
• 作者: Jun YAN;Yukio AIZAWA;Jiro HITOMI
• 通讯作者: Jiro HITOMI

Localization of motoneurons the extend axons through the ventral rami of cervical nerves to innervate the trapezius muscle : A study using fluorescent dyes and 3D reconstruction method

运动神经元的定位，通过颈神经腹侧支延伸轴突来支配斜方肌：使用荧光染料和 3D 重建方法的研究

• 发表时间: 2007
• 期刊: Clinical Anatomy 20
• 作者: Jun Y;Yukio A;Jiro H
• 通讯作者: Jiro H