The corresponding relationship between t he changes of the motor neurons after peripheral nerve transfer and the effects of peripheral nerve regeneration

周围神经移植后运动神经元的变化与周围神经再生效果的对应关系

• 批准号: 22590178
• 负责人: YAN Jun
• 金额: $ 3万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590178/
• 关键词: Nerve Transfer Treatment; Fluorescent Dye Labeling; Electromyogram; Nerve Axonal Regeneration; Rat; 末梢神経移植; α運動ニューロン; γ運動ニューロン; 副神経; 肩甲上神経; Nerve Transfer; Fluorescent Dye; EMG; Regeneration; 臨床解剖学; 末梢神経再生; 運動ニューロン; 筋電図; 逆行性標識

For many years, nerve transfer has been commonly used as a treatment option following peripheral nerve injury, although the precise mechanism underlying successful nerve transfer is not yet clear. We developed an animal model to investigate the mechanism underlying nerve transfer between branches of the spinal accessory nerve (Ac) and suprascapular nerve (Ss) in rats, so that we could observe changes in the number of motor neurons, investigate the 3 -dimensional localization of neu-rons in the anterior horn of the spinal cord, and perform an electromyogram (EMG) of the suprasp -inatus muscle before and after nerve transfer treatment. The present experiment showed a clear re-duction in the number of γmotor neurons. The distributional portion of motor neurons following nerve transfer was mainly within the neuron column innervating the trapezius. Some neurons in-nervating the supraspinatus muscle also survived post-transfer. Compared with the non-operated group, the EMG restoration rate of the supraspinatus muscle following nerve transfer was 60% in the experimental group and 80% in a surgical control group. Following nerve transfer, there was a distinct reduction in the number of γmotor neurons. Therefore, γmotor neurons may have important effects on the recovery of muscular strength following nerve transfer. Moreover, because the neurons located in regions innervating either the trapezius or supraspinatus muscle were labeled after Ac transfer to Ss, we also suggest that indistinct axon regeneration mechanisms exist in the spinal cord following peripheral nerve transfer.

多年来，神经转移通常被用作外周神经损伤后的治疗选择，尽管成功的神经转移的精确机制尚不清楚。我们开发了一种动物模型，以研究大鼠的脊柱饰品神经（AC）和上次神经神经（SS）之间神经转移的基础机制，以便我们可以观察到运动神经元数量的变化，研究脊髓前角的3维神经元的3维定位，并在脊髓的前角和emg ersistraus and emprace and the emprace -insus -insus -insus -insus -insus -inseraus -Ins ersus -in In In In。本实验显示γMotor神经元的数量明显减少。神经传递后运动神经元的分布部分主要在神经trapezius的神经元柱中。一些神经元引起了肌肉上肌的影响，在转移后也幸存下来。与非手术组相比，神经转移后肌肉上肌的EMG恢复速率在实验组中为60％，手术对照组为80％。神经转移后，γMotor神经元的数量明显减少。因此，γMOTOR神经元可能对神经转移后肌肉强度的恢复具有重要影响。此外，由于位于AC转移到SS后的斜方肌或上肌上的区域中的神经元被标记为标记，因此我们还建议在周围神经转移后脊髓中存在模糊的轴突再生机制。

项目成果

脊髄前角ニューロンの形態学的解析と複合筋電図によるラット副神経僧帽筋枝-肩甲上神経移行術の評価、

脊髓腹角神经元形态学分析和复合肌电图评价大鼠副神经斜方支-肩胛上神经转移技术

• 发表时间: 2010
• 期刊: J Iwate Med Associa
• 作者: 荻野和仁;燕 軍
• 通讯作者: 燕 軍

脊髄前角ニューロンの形態学的解析と複合筋電図によるラット副神経僧帽筋枝-肩甲上神経移行術の評価

脊髓腹角神经元形态学分析和复合肌电图评价大鼠副神经斜方支-肩胛上神经转移技术

• 发表时间: 2010
• 期刊: J Iwate Med Associa
• 作者: 荻野和仁;燕軍
• 通讯作者: 燕軍

僧帽筋支配枝と肩甲上神経に含まれる各ニューロンの数と局在の同定

识别斜方肌神经支配分支和肩胛上神经中每个神经元的数量和定位

• 发表时间: 2009
• 作者: 荻野和仁;燕 軍;佐々木航;人見次郎
• 通讯作者: 人見次郎

Morphological and electromyogram analysis for the spinal accessory nerve transfer to the suprascapular nerve in rats

大鼠脊髓副神经向肩胛上神经转移的形态学和肌电图分析

• DOI: 10.4236/ss.2011.25059
• 发表时间: 2011
• 期刊: Surgical Science
• 作者: Jun Yan;Kazuhito Ogino;Jiro Hitomi
• 通讯作者: Jiro Hitomi

ラット胸鎖乳突筋・僧帽筋の二重神経支配の再検討

大鼠胸锁乳突肌和斜方肌双重神经支配的复查

• 发表时间: 2010
• 作者: Jun Yan;Wataru Sasaki;燕 軍
• 通讯作者: 燕 軍