Identification and characterization of centrosome re-duplication suppressor gene(s)
中心体重复复制抑制基因的鉴定和表征
基本信息
- 批准号:17590286
- 负责人:
- 金额:$ 1.66万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2005
- 资助国家:日本
- 起止时间:2005 至 2006
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The aim of this study is to identify centrosome re-duplication suppressor gene(s) by chromosome-transfer technology. We carried out the following experiments.(1) At first, we analyzed centrosome status of mouse A9 cells. Mouse A9 cells are normal origin but makes tumors in nude mice. Thus, A9 cells are precancerous cell line. We examined centrosome staus in A9 cells by immunostaining, and found that centrosome number is normal. We next expose hydroxyurea (HU), a DNA synthesis inhibitor, to A9 cells. The exposed cells were arrested at G1-S transition in cell cycle and were inhibited DNA synthesis. However, centrosome duplication cycle was dysregulated in A9 cells; multi-round of centrosome duplication occurred under HU treatment. Thus, A9 cells have a potent centrosome re-duplication activity.(2) Next, we tried to examine centrosome re-duplication activity in A9 cells transferred with a human chromosome 1, 5, 8, 10, 11, 13, 15, 16, 17, and 18, respectively. As a result, we found that centrosome re-duplication activity was suppressed in A9-Ch. 8, A9-Ch. 15, and A9-Ch. 17 cells, respectively. These results suggest that the gene(s), which is related in suppression of centrosome re-duplication, is located on chromosome 8, chromosome 15, and chromosome 17. Especially, A9-Ch. 8 cells have high centrosome re-duplication suppressor activity. Therefore, we determined to isolate centrosome re-duplication suppressor gene(s) from chromosome 8.(3) As it is found that human osteosarsoma cell line, U2OS has centrosome re-duplication activity, we transferred chromosome 8 into U2OS cells and examined centrosome re-duplication activity. As a result, we found that chromosome 8 had a centrosome re-duplication suppressor activity. Now, we tried to isolate the candidate gene by radiation hybrid mapping.
本研究的目的是利用染色体转移技术鉴定中心体重复抑制基因。我们进行了以下实验。(1)首先,我们分析了小鼠A9细胞的中心体状态。小鼠A9细胞是正常来源,但在裸鼠中产生肿瘤。因此,A9细胞是癌前细胞系。我们用免疫组化方法检测了A9细胞的中心体状态,发现中心体数目正常。接下来,我们将DNA合成抑制剂羟基脲(HU)暴露于A9细胞。细胞周期阻滞于G1-S期,DNA合成受到抑制。然而,在A9细胞中,中心体复制周期失调; HU处理下发生多轮中心体复制。因此,A9细胞具有有效的中心体再复制活性。(2)接下来,我们试图检查分别用人染色体1、5、8、10、11、13、15、16、17和18转移的A9细胞中的中心体再复制活性。结果,我们发现,中心体再复制活动被抑制在A9-Ch.8,A9-Ch.15,和A9-Ch.17细胞,分别。这些结果表明,与抑制中心体重复有关的基因位于8号、15号和17号染色体上。特别是A9-Ch.8细胞具有高的中心体重复抑制活性。因此,我们决定从8号染色体上分离中心体再复制抑制基因。(3)由于发现人骨肉瘤细胞系U2 OS具有中心体再复制活性,我们将8号染色体转入U2 OS细胞中,并检测其中心体再复制活性。结果,我们发现8号染色体具有中心体重复抑制活性。现在,我们试图通过辐射杂交定位来分离候选基因。
项目成果
期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
中心体複製機構と染色体異数性との関連:第十二回臨床細胞遺伝学セミナーテキスト
中心体复制机制与染色体非整倍体的关系:第十二届临床细胞遗传学研讨会正文
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Matsuura;S.;et al.;Arinami T;Ryuji Ikeda;泉 秀樹
- 通讯作者:泉 秀樹
Monoallelic BUB1B mutations and defective mitotic-spindle checkpoint in seven families with premature chromatid separation (PCS) syndrome
- DOI:10.1002/ajmg.a.31069
- 发表时间:2006-02-15
- 期刊:
- 影响因子:2
- 作者:Matsuura, S;Matsumoto, Y;Kajii, T
- 通讯作者:Kajii, T
Monoallelic BUB1B mutations and defect mitotic spindle checkpoint in seven families with premature chromatid separation syndrome.
七个染色单体过早分离综合征家系的单等位基因 BUB1B 突变和有丝分裂纺锤体检查点缺陷。
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Matsuura;S.;et. al.;Maehara et al.;Arinami T;Matsumura Shinya et al.
- 通讯作者:Matsumura Shinya et al.
Mortalin controls centrosome duplication via modulating centrosomal localization of p53
- DOI:10.1038/sj.onc.1209543
- 发表时间:2006-08-01
- 期刊:
- 影响因子:8
- 作者:Ma, Z.;Izumi, H.;Fukasawa, K.
- 通讯作者:Fukasawa, K.
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IZUMI Hideki其他文献
IZUMI Hideki的其他文献
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{{ truncateString('IZUMI Hideki', 18)}}的其他基金
Trim32-based new differentiation therapy for neuroblastoma
基于 Trim32 的神经母细胞瘤新分化疗法
- 批准号:
15K09674 - 财政年份:2015
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular cloning of the gene on chromosome 8, which suppresses centrosome re-duplication
8号染色体上基因的分子克隆,抑制中心体重复
- 批准号:
19590334 - 财政年份:2007
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
Molecular cloning of the gene on chromosome 8, which suppresses centrosome re-duplication
8号染色体上基因的分子克隆,抑制中心体重复
- 批准号:
19590334 - 财政年份:2007
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)