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Molecular regulation and mechanism of differentiation and transformation of Trichinella infected muscle cell

旋毛虫感染肌细胞分化转化的分子调控及机制

基本信息

批准号：
17590370
负责人：
WU Zhiliang
金额：
$ 2.24万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

cDNA microarray analysis and comparison of the gene expression profiles between Trichinella spiralis and T. pseudospiralis infected muscle tissues were performed to reveal the molecular mechanism of the pathological changes and nurse cell formation. The candidate genes to be likely involved in the common and different pathological changes were listed. The expressions of the genes associated with satellite cell activation and proliferation, Pax7, desmin and M-cadherin, were up-regulated, suggesting their involvement in the roles of satellite cells during the formation of nurse cell. Differential expression of the genes related to cell differentiation was observed, for example, up-regulated expression of galectin and Pbxl in both Trichinella infections, up-regulated of Pax3, IFI202a, chordin 2 and S100A9, and down-regulated of Ndrg2 only in T. spiralis infection, and up-regulated of ATBF1, FoxH1, Rfx3 and Sufu, and down-regulated of Nanog only in T. pseudospiralis infection. Several gene … More s to regulate cell cycle and proliferation showed changes in expression after infection, for example, G0S2, CLU, MLF1, Id2, B-myb and cyclin A2, that are likely to play role in cell cycle reentry and arrest occurred during infection. The differential expression of some genes associated with apoptosis and transformation was identified. Further confirmation of the expression and kinetics of some selected genes by real time PCR revealed the different patterns between the two Trichinella infections. In addition, two signaling pathways, TGF-β and Notch signaling pathways, were confirmed to be involved in the differentiation and transformation of infected muscle cell. The present results indicated that complex pathological processes induced by Trichinella infection were regulated by many genes and signaling pathway, mainly related to muscle development, myogenesis, cell proliferation, differentiation and transformation, cell cycle regulation, and apoptosis. The differential expression of some genes is likely to be responding to different pathology between T spiralis and T pseudospiralis infection. Further analysis of some key ones of the listed candidate genes will help to reveal the molecular mechanism of nurse cell formation. Less

旋毛虫和旋毛虫基因表达谱的cDNA微阵列分析和比较目的：研究假螺旋体感染肌肉组织的病理变化及滋养细胞形成的分子机制。列出了可能参与常见和不同病理变化的候选基因。与卫星细胞活化和增殖相关的基因Pax 7、desmin和M-cadherin的表达上调，提示它们参与了卫星细胞在滋养细胞形成过程中的作用。在旋毛虫感染后，Galectin和Pbxl基因表达上调，Pax 3、IFI 202 a、chordin 2和S100 A9基因表达上调，而Ndrg 2基因表达下调。感染旋毛虫后，ATBF 1、FoxH 1、Rfx 3和Sufu表达上调，Nanog表达下调。假螺旋体感染几个基因 ...更多信息感染后，G 0 S2、CLU、MLF 1、Id 2、B-myb和cyclin A2等基因表达发生变化，可能参与了感染后细胞周期的重新进入和停滞。鉴定了一些与凋亡和转化相关的基因的差异表达。通过真实的时间PCR进一步证实了一些选择的基因的表达和动力学，揭示了两种旋毛虫感染之间的不同模式。另外，两条信号通路TGF-β和Notch信号通路被证实参与了感染肌细胞的分化和转化。这些结果表明旋毛虫感染诱导的复杂病理过程受多种基因和信号通路的调控，主要涉及肌肉发育、肌形成、细胞增殖、分化和转化、细胞周期调控和细胞凋亡等。某些基因的差异表达可能是旋毛虫和假旋毛虫感染的不同病理反应。对其中一些关键基因的进一步分析将有助于揭示滋养细胞形成的分子机制。少