Immunological and structural analysis of immunosuppressive effect of excretory-secretory protein from Trichinella pseudospiralis

我们先前的研究表明，旋毛虫感染可以通过抑制Th17和Th1反应来改善自身免疫性脑脊髓炎。本实验研究了重组伪旋毛虫53 kDa排泄分泌蛋白(TP53)的免疫调节作用，包括对巨噬细胞产生IL-6和脾细胞产生IL-17、干扰素-γ和IL-4的影响。结果提示，重组TP53具有抑制巨噬细胞产生IL-6，抑制脾细胞Th17和Th1应答的免疫调节功能，提示TP53通过抑制IL-6和IL-17正反馈环而抑制Th17应答。我们已经开始了TP53的结晶。然而，我们还没有得到任何晶体。

Our previous study indicated that Trichinella pseudospirlais infection can ameliorate autoimmune encephalomyelitis through suppressing Th17 and Th1 responses. In the present study, we investigated the immunomodulatory function of the recombinant T. pseudospiralis 53kDa excretory-secretory protein (Tp53), including the effect on the production of IL-6 of macrophage and IL-17, IFN-gamma and IL-4 of splenocyte in vitro and in vivo. Results suggested the recombinant Tp53 has immune regulatory function of inhibiting IL-6 production of macrophage, and suppressing Th17 and Th1 responses of splenocyte, suggesting Tp53 possesses immunomodulation function to suppress Th17 response via inhibiting IL-6 and IL-17 positive feedback loop.For structural studies, we have established expression and purification system using E. coli and pCold-GST vector. We have started the crystallization of Tp53. However, we have not obtain any crystal yet.