Proteome analysis of cardiomyocyte proteins in forensic autopsy cases and application to forensic pathological diagnosis

我们对心肌细胞蛋白的表达变化进行了差异蛋白质组学分析研究；二维凝胶电泳检测法医尸检病例左心室肌中聚乳酸蛋白、肌钙蛋白T、凝胶蛋白、α-平滑肌肌动蛋白（α-SMA）、锰超氧化物歧化酶（MnSOD）和α-烯醇化酶的含量[j]。Desmin: Desmin异常见于心肌病和心力衰竭病例。窒息组与心源性猝死组（p<0.03）、颅脑损伤组（p<0.02）、出血组与颅脑损伤组（p<0.05）在磷酸化与去磷酸化desmin比值上差异均有统计学意义。Gelsolin: Gelsolin是一种丰富的肌动蛋白结合蛋白，心肌病患者的Gelsolin蛋白表达增加。结果表明，有16个凝胶点的等电点（pI）向酸性区转移。肌钙蛋白T：心肌肌钙蛋白T是心脏收缩调节和心肌肌丝对钙的敏感性的重要组成部分。我们检测到肌钙蛋白T点的改变除了原来的点在5。在6例病例中，我们找不到肌钙蛋白T点。α-SMA：在实验大鼠模型中，增生性刺激诱导“胎儿基因”的早期表达，包括α-SKA和α-SMA。将51例法医尸检病例α-SMA的表达分为3组：1. α-SMA的表达；强（n=9）， 2。中度（n=21）；弱或阴性（n=21）。MnSOD: MnSOD是保护心脏免受缺血再灌注损伤的主要抗氧化酶之一。19例在基本区检出新的MnSOD斑点。α-烯醇化酶：α-烯醇化酶是一种糖酵解酶，在缺血再灌注损伤大鼠心脏中观察到该酶的表达。我们在19例患者（包括4例心源性猝死）中证实了α-烯醇化酶的两倍过表达。

We performed a differential proteomic profiling study on expression changes of cardiomyocyte proteins ; desmin, troponin T, gelsolin, α-smooth muscle actin (α-SMA), manganese superoxide dismutase (MnSOD) and α-enolase in left ventricular muscles of forensic autopsy cases by two-dimensional gel electrophoresis.1.Desmin : Des min abnormalities were reported in cardiomyopathy and heart failure cases. The significant differences were found between asphyxia and sudden cardiac death (p<0.03), and head injury (p<0.02), between bleeding and head injury (p<0.05) in the ratio of phosphorylated to dephosphorylated desmin.2. Gelsolin : Gelsolin is an abundant actin binding protein and the protein expression was increased in cardiomyopathic case. We demonstrate that isoelectric point (pI) of several gelsolin spots shifted to acidic region in 16 cases.3. Troponin T: Cardiac troponin T is an essential component of the cardiac contraction regulations and the sensitivity of cardiac myofilaments to calcium. We detected altered troponin T spots in addition to original spots in 5. In 6 cases, we couldn't find the tropnin T spots.4. α-SMA : In experimental rat models, hypertrophic stimuli induce early expression of 'fetal genes' including α-SKA and α-SMA. The expression of α-SMA in 51 forensic autopsy cases was divided into 3 groups : 1. Strong (n=9), 2. Moderate (n=21), 3. Weak or Negative (n=21).5. MnSOD : MnSOD is one of the main antioxidant enzymes that protect the heart against ischemia-reperfusion injury. In 19 cases, a new MnSOD spot was detected in basic region.6. α-Enolase : α-Enolase is a glycolytic enzyme and the enzyme expression is observed in rat heart subjected ischemia-reperfusion injury. We demonstrated over a two-fold overexpression of α-enolase in 19 cases including four sudden cardiac death cases.