Determination of ABO blood group of seminal stain mixed with vaginal secretion using immunoblotting

本研究的目的是开发一种新技术，用于确定性病例中精液和阴道液的每个ABO血型。从人精浆中分离到一种ABO血型糖蛋白，命名为“slpha_2-半糖蛋白（alpha_2-SGP）”。用完全弗氏佐剂乳化的alpha_2-SGP免疫家兔制备抗alpha_2-SGP血清。建立了以抗α _2- sgp血清、抗A、抗b血清为检测对象，采用SDS-PAG电泳-免疫印迹法测定精液和阴道液混合液中各ABO血型的新方法。(1)阴道液中不能检测到alpha_2-SGP。这一结果提示alpha_2-SGP是一种精ABO血型特异性糖蛋白。(2) ABO血型α _2- sgp活性与阴道液重叠；因此不能区分精液和阴道液的ABO血型。结果表明，alpha_2-SGP可作为测定精液ABO血型的有效标记物。然而，这种方法并不适用于法医学案件。

A purpose of this research is development of a new technique for the determination of each ABO blood group of semen and vaginal fluid in sexual cases.An ABO blood group glycoprotein, which was designated as "slpha_2-seminoglyco- protein (alpha_2-SGP)" was isolated from human seminal plasma. The anti-alpha_2-SGP sera were prepared by immunizing rabbits with the alpha_2-SGP emulsitied with complete Freund's adjuvant.A new method for determination of each ABO blood group of semen and vaginal fluid in mixture of both semen and vaginal fluid by SDS-PAG electrophoresis-Immunoblotting using anti-alpha_2-SGP sera, anti-A and anti-B was carried out.(1) The alpha_2-SGP cannot be detected in vaginal fluid. This result suggests that alpha_2-SGP is a seminal ABO blood group specific glycoprotein.(2) ABO blood group activity of alpha_2-SGP overlaps with that of vaginal fluid ; therefore each ABO blood group of semen and vaginal fluid could not be distinguished.Results show alpha_2-SGP to be a useful blood group marker for the determination of ABO blood group of semen. However, this method was found to be unsuitable for use with forensic science case.