Fundamental examination about the prevention / treatment of digestive organs disease by Tre foil peptide

本研究是生产生活和分泌性的，旨在探讨与消化道粘膜角质层具有较强的消化道粘膜保护作用的托盘箔肽（TFF）临床应用的可能性。我们进行了分析，考虑到临床应用的TFF诱导剂，并检查了各种原因，特别是提高内源性TFF表达的孩子。对人类来说，它是PPARγ；由于只有人类TFF2基因才允许在TFF2基因上游阵列中出现PPAR反应区，因此在胃粘膜上皮细胞中发现PPARγ通过Rigand诱导TFF2的出现；在人体和动物实验模型中，对胃粘膜保护的作用进行了思考，认为其结果可能造成不可逾越的鸿沟。由于已知雌激素在其他细胞系统中发挥着TFF1外观的中心作用，例如在有TFF2的胃粘膜中出现的关于TFF1的乳腺癌细胞，因此我们对雌激素对胃中TFF1外观的影响进行了详细的研究。结果表明，虽然胃粘膜上皮细胞中出现了雌激素受体（ER）（主要是ERβ），但雌激素对TFF1的表达没有影响。此外，NE-κB；研究了用B刺激的Cigna环对TFF1外观的影响，发现TFF1的外观水平是TNF-α，它与炎症细胞因子一起升高。此外，由于计划在体内使用Ricombinant TFF肽治疗或尝试TFF基因导入治疗，因此对TNBS致结肠炎模型等动物实验模型进行了初步检查。

This research is a production life and is secreted, and has aimed to examine the possibility of a clinical application of tray foil peptide (TFF) that has a strong digestive tube mucous membrane protection action with the digestive tube mucous membrane cuticle. We performed the analysis that took clinical application into consideration of TFF inducer and examined in particular the various reasons child who raised endogenous TFF expression. For the human, it is PPARγ ; Because it is only a human TFF2 gene that the PPAR response area is admitted in the TFF2 gene upstream array, PPARγ though Rigand had been found inducing the appearance of TFF2 in the stomach mucous membrane epithelial cell ; The possibility that the unbridgeable gulf is caused in the result in the human and the animal experiment model was thought about the effect of Rigand of the stomach mucous membrane protection. Because it was known that estrogen played TFF1 appearance a center role in other cell systems such as the breast cancer cells about TFF1 that appeared high by the gastric mucosa with TFF2, a detailed examination was done about the influence of estrogen on the TFF1 appearance in the stomach. As a result, estrogen's not influencing the TFF1 appearance at all though estrogen receptor (ER) appeared in the stomach mucous membrane epithelial cell (chiefly ERβ) became clear. Moreover, NE-κB ; The influence that the Cigna ring of the inflammation stimulation for which B is used exerts on the TFF appearance is examined, and the appearance level of TFF1 is TNF-α It had been found to rise with an inflammatory cytokine. In addition, because treatment with Ricombinant TFF peptide in in vivo or the attempt of the TFF gene introduction treatment was scheduled, the animal experiment model's like TNBS cause colitis model etc. preliminary examinations were done.