The evaluation of cultured complexed periosteal cell graft fora repair of bone defect.

骨膜具有成骨潜能，其作为骨和关节缺损修复的移植材料已受到相当大的关注。近年来，有关骨膜细胞体外培养的成骨潜能的研究已有报道。我们目前的研究结果诱导骨形成从培养的骨膜来源的细胞大鼠胫骨。骨膜具有成骨潜能，作为骨和关节缺损修复的移植材料已受到相当大的关注。培养的骨膜细胞的成骨潜能也有报道。在这里，使用异种移植大鼠模型，从培养的人骨膜来源的细胞诱导骨形成的研究结果。将人下颌骨骨膜置于含10%胎牛血清的培养基中培养14天。达到汇合后，骨膜细胞用0.25%胰蛋白酶/EDTA重悬，然后在胶原海绵上三维再培养。将骨膜细胞/胶原复合物移植到大鼠颅骨中，并肌内注射免疫抑制剂（FK 506，1.0 mg/kg/天）。在术后14天和28天，移植组织被摘除，并与仅胶原移植组的组织进行组织学和放射学比较。实验组骨膜细胞在移植后14天开始增殖并分化为成骨细胞。第28天，新骨形成明显。到第7天，观察到骨生长，并在缺损处检测到新的钙化。培养的人骨膜来源的细胞在异种移植模型中表现出成骨潜能，使用大鼠颅骨缺损。培养的骨膜细胞诱导成骨不充分。对于临床应用，我们需要更多的实验研究来促进骨形成。

Periosteum has osteogenic potential and considerable attention has been focused on its use as a grafting material for the repair of bone and joint defects. Recently, the osteogenic potential of cultured periosteal cells has been reported. We present findings of bone formation induced from cultured periosteum-derived cells of rats tibiae. Periosteum shows osteogenic potential and has received considerable attention as a grafting material for the repair of bone and joint defects. The osteogenic potential of cultured periosteal cells has also been reported. Here the findings of bone formation induced from cultured human periosteum-derived cells using a xenogeneic graft rat model are presented. Human mandibular periosteum was placed into a culture medium with 10% fetal bovine serum for 14 days. After reaching confluence, periosteal cells were resuspended with 0.25% trypsin/EDTA and then re-cultured three dimensionally on a collagen sponge. The periosteal cell/collagen complex was grafted into rat calvarial rats and an immunosuppressant (FK506, 1.0 mg/kg/day) was administered intramuscularly. At 14 and 28 days postoperatively, grafted tissue was extirpated and compared histologically and radiographically with tissue from a collagen-only grafted group. In the experimental group, periosteal cells had proliferated and differentiated into osteogenic cells by 14 days post grafting. At 28 days, new bone formation was evident. By 7 days, bone growth was observed and new calcification was detected in the defect. Cultured human periosteum-derived cells showed osteogenic potential in a xenogeneic graft model using rat calvarial defects. The bone formation induced from cultured periosteal cells were not sufficient. For clinical use, we need more experimental research to prompt bone formation.