The evaluation of cultured complexed periosteal cell graft fora repair of bone defect.

培养复合骨膜细胞移植修复骨缺损的评价。

• 批准号: 17592079
• 负责人: UENO Takaaki
• 金额: $ 2.11万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592079/
• 关键词: Perio steal graft; Blood vessels formation; Bone regeneration; Mandibular repair; β-TCP; ヒト骨膜細胞; 培養; 移植; 骨再生

Periosteum has osteogenic potential and considerable attention has been focused on its use as a grafting material for the repair of bone and joint defects. Recently, the osteogenic potential of cultured periosteal cells has been reported. We present findings of bone formation induced from cultured periosteum-derived cells of rats tibiae. Periosteum shows osteogenic potential and has received considerable attention as a grafting material for the repair of bone and joint defects. The osteogenic potential of cultured periosteal cells has also been reported. Here the findings of bone formation induced from cultured human periosteum-derived cells using a xenogeneic graft rat model are presented. Human mandibular periosteum was placed into a culture medium with 10% fetal bovine serum for 14 days. After reaching confluence, periosteal cells were resuspended with 0.25% trypsin/EDTA and then re-cultured three dimensionally on a collagen sponge. The periosteal cell/collagen complex was grafted into rat calvarial rats and an immunosuppressant (FK506, 1.0 mg/kg/day) was administered intramuscularly. At 14 and 28 days postoperatively, grafted tissue was extirpated and compared histologically and radiographically with tissue from a collagen-only grafted group. In the experimental group, periosteal cells had proliferated and differentiated into osteogenic cells by 14 days post grafting. At 28 days, new bone formation was evident. By 7 days, bone growth was observed and new calcification was detected in the defect. Cultured human periosteum-derived cells showed osteogenic potential in a xenogeneic graft model using rat calvarial defects. The bone formation induced from cultured periosteal cells were not sufficient. For clinical use, we need more experimental research to prompt bone formation.

骨膜具有成骨潜能，作为骨和关节缺损区的移植材料已引起广泛关注。最近，培养的骨膜细胞具有成骨潜能的报道。我们报道了培养的大鼠胫骨骨膜来源的细胞诱导骨形成的研究结果。骨膜具有成骨潜能，作为骨和关节缺损区的移植材料受到了广泛的关注。培养的骨膜细胞的成骨潜能也已有报道。本文介绍了利用异种移植大鼠模型从培养的人骨膜来源的细胞诱导骨形成的研究结果。将人下颌骨骨膜置于含10%胎牛血清的培养液中培养14天。骨膜细胞达到融合后，再用0.25%胰酶/EDTA悬浮，然后在胶原海绵上进行三维再培养。将骨膜细胞/胶原复合体移植到大鼠颅骨内，肌肉注射免疫抑制剂FK506(1.0 mg/kg/d)。在术后14天和28天，摘除移植组织，并在组织学和放射学上与单纯胶原移植组的组织进行比较。实验组移植后14d骨膜细胞已增殖分化为成骨细胞。28天时，新骨形成明显。7天后，观察到骨生长，并在缺损处发现新的钙化。培养的人骨膜来源的细胞在使用大鼠颅骨缺损的异种移植模型中显示出成骨潜力。体外培养的骨膜细胞诱导成骨能力不强。对于临床应用，我们还需要更多的实验研究来促进骨形成。

项目成果

The histological and radiographical evaluation of beta-tricalciumphosphate Barcelona, for dental implants requiring bone augmentation.

β-磷酸三钙巴塞罗那的组织学和放射学评估，用于需要骨增量的牙种植体。

• 发表时间: 2006
• 期刊: XVIII Journal of CranioMaxillofac Surg. Proceeding. Congress of the European Association for Cranio-Maxillofacial Surgery. International Proceedings.
• 作者: Takaaki Ueno;Kanou;Kozo Honda Toshio Sugahara.
• 通讯作者: Kozo Honda Toshio Sugahara.

β-TCP(Tricalcium phosphate) を用いた顎骨増量術のインプラント治療への臨床的検討

使用β-TCP（磷酸三钙）进行颌骨增量种植治疗的临床考虑

• 发表时间: 2006
• 期刊: 岡山歯雑誌 25
• 作者: 本多浩三;植野高章;坂田吉朗;脇本真理;他
• 通讯作者: 他

Expression of transcription factor Sox9 in the cartilage formation from grafted periostel cells

转录因子Sox9在移植骨膜细胞软骨形成中的表达

• 发表时间: 2006
• 期刊: Asian Journal Oral Maxillofac Surg 18
• 作者: Takaaki Ueno;Toshimasa Kagawa;Miwa Kanou;et al.;Toshiro Sakata 他5名;Takaaki Ueno 他6名
• 通讯作者: Takaaki Ueno 他6名

Clinical Effects of Cevimeline Hydrochloride for the Treatment of Dry Mouth in 2 patients with Sjogren's Syndrome

盐酸西维美林治疗干燥综合征口干2例临床疗效

• 发表时间: 2005
• 期刊: Journal of hard tissue biology 14(2)
• 作者: Cui Z.-G.;et al.;K.NAGATA et al.;Takeda Y;Yamachika E;Mizukawa N;山村 功ら;Tsujigiwa H;Fukunaga J;Takagi S;Nagatsuka H;Takagi S;Nobuhisa T;Nobuyoshi Mizukawa
• 通讯作者: Nobuyoshi Mizukawa

Immunohistochemical analysis of Sox9 in the periosteum of tibia nad calvaria after surgical release of periosteum

骨膜松解术后胫骨和颅骨骨膜中 Sox9 的免疫组织化学分析

• 发表时间: 2005
• 期刊: Acta Histochemica 106
• 作者: Fujii T;Ueno T;Kagawa T;Kanou M;Fukunaga J;Sugahara T.
• 通讯作者: Sugahara T.