Plasma-modified peptides/proteins for multi-target anticancer treatment

用于多靶点抗癌治疗的血浆修饰肽/蛋白质

• 批准号: 22H01212
• 负责人: Attri Pankaj
• 金额: $ 11.07万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22H01212/
• 关键词: Computational study; Protein; Plasma Chemistry; Nitrogen conversion; Plant protein; COMSOL Multiphysics; CO2 and N2 plasma; Fluorescence; Plasma agriculture; plasma medicine; peptides; proteins; anticancer treatment

We developed steamer discharge plasma working on N2, CO2, and Air feed gas. We also developed reaction chemistry for CO2, N2, and Air plasma to understand the production of short-lived species. Our 0D and 1D models are ready nowadays; we are focused on developing 2D models. 2D models in two different phases took a very long time to standardize due to complications in fluid flow in two distinct phases. Our 2D model is very close to the experimental conditions of the gas and liquid phase. OD and 1D model COMSOL model were helpful in predicting the very short reactive species those were are not possible to detect through the physical parameters. like We also measured the reactive species in the gas and liquid phase. Further, we measured the changes in the reactive species using a chemical analysis Kit and Fluorescence spectroscopy for the liquid phase analysis, and FT-IR used for the gas phase analysis. We analyzed our plasma system on the radish seeds to find out the change in germination index and growth. Also new analyzed the production of the NO3- species inside the seeds. We also checked the production of reactive production in the gas phase like CO, and O3 using FTIR. And detected the NH4+, NO3-, NO2- and H2O2 concentrations in the water using chemical analysis. Later, we also checked the production of OH radical in the water using the Fluorescence spectroscopy. The detection of OH, N2 second positive system, NO, CO, Alpha-H were detected in the gas phase using Optical emission spectroscopy. Our Simulation results are in the good agreement with the experimental results

我们开发了蒸汽放电等离子体工作在N2，CO2和空气原料气。 我们还开发了CO2，N2和空气等离子体的反应化学，以了解短寿命物种的产生。我们的0D和1D模型已经准备就绪;我们专注于开发2D模型。由于两个不同阶段中流体流动的复杂性，两个不同阶段中的2D模型需要很长时间来标准化。我们的二维模型非常接近气相和液相的实验条件。OD和一维模型COMSOL模型有助于预测那些通过物理参数无法检测到的非常短的反应物种。我们还测量了气相和液相中的活性物质。此外，我们使用化学分析试剂盒和荧光光谱法进行液相分析，并使用FT-IR进行气相分析，以测量活性物质的变化。我们分析了我们的等离子体系统对萝卜种子的发芽指数和生长的变化。还新分析了种子内NO3-物质的产生。我们还使用FTIR检查了气相中反应产物如CO和O3的产生。采用化学分析法测定了水体中NH4+、NO3-、NO2-和H2O2的浓度。随后，我们还使用荧光光谱检查了水中OH自由基的产生。采用光发射光谱法对气相中的OH、N2二正体系、NO、CO、α-H进行了检测。模拟结果与实验结果吻合较好

项目成果

Outcomes of Pulsed Electric Fields and Nonthermal Plasma Treatments on Seed Germination and Protein Functions

• DOI: 10.3390/agronomy12020482
• 发表时间: 2022-02-01
• 期刊: AGRONOMY-BASEL
• 影响因子: 3.7
• 作者: Attri, Pankaj;Okumura, Takamasa;Takaki, Koichi
• 通讯作者: Takaki, Koichi