Thyroid hormones and Pigmentation pattern evolution in clownfish

小丑鱼的甲状腺激素和色素沉着模式的演变

• 批准号: 22H02678
• 负责人: LAUDET Vincent
• 金额: $ 11.07万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22H02678/
• 关键词: Pigmentation; Clownfish; Thyroid Hormone; Ontogenesis

How differences in pigmentation arose have never been fully explained. In anemonefishes there is a difference in white bar formation between Amphiprion ocellaris and A. percula. As white bars are linked to thyroid hormones (TH) we study if this ontogeny difference is linked to differences in TH signaling. We collected samples of the 2 species from hatching until 100 days. We have added the closely related A. biaculeatus to determine the ancestral status of bar formation. The samples are ready and will soon be sent for Illumina sequencing. We treated A. ocellaris with thyroid hormone and to MPI (to block TH production) and we submitted the samples to transcriptomic analysis. We have established the CRISPR/Cas9 technology in A. ocellaris and have targeted a first gene (dio2).

色素沉着差异是如何产生的从未得到充分解释。在银鱼中，眼斑双锯鱼和银鱼的白色条形成有差异。percula。由于白色条与甲状腺激素（TH）有关，我们研究这种个体发育差异是否与TH信号传导的差异有关。我们从孵化到100天收集了这两个物种的样品。我们已经添加了密切相关的A。biaculeatus，以确定酒吧形成的祖先地位。样品已经准备好，很快将被送往Illumina测序。我们治疗了A。我们用甲状腺激素和MPI（阻断TH产生）处理单眼细胞，并将样品提交给转录组学分析。我们已经在A. ocellaris并靶向第一基因（d10 2）。

项目成果

Megacolonies: an alternative social organization in anemonefishes?

巨群落：小丑鱼的另一种社会组织？

• DOI: 10.1101/2022.11.06.515354
• 发表时间: 2022
• 期刊: bioRxiv
• 作者: Mercader Manon;Zwahlen Jann;Hayashi Kina;Takamiyagi Hiroki;Tseng Yung-Che;Nguyen Hai-Thanh T.;Asada Keishu;Sowinski J?rome;Reimer James;Lecchini David;Laudet Vincent
• 通讯作者: Laudet Vincent

Evolution, Development and Ecology of Anemonefishes. Model Organisms for Marine Science

海葵鱼的进化、发育和生态学。

• 发表时间: 2022
• 作者: 髙橋宗春;戸田景子;林田美緒;Huijia Wang;川原玄理;林由起子;Laudet V and Ravasi T
• 通讯作者: Laudet V and Ravasi T