Thyroid hormones and Pigmentation pattern evolution in clownfish

色素沉着的差异是如何产生的一直没有得到充分的解释。在海葵中，眼镜蛇和海葵在白条的形成上存在差异。由于白条与甲状腺激素(TH)有关，我们研究这种个体发育差异是否与TH信号的差异有关。我们收集了这两个物种从孵化到100日龄的样本。我们添加了与其亲缘关系密切的双棘豆，以确定沙洲形成的祖先地位。样品已经准备好，很快就会被送去进行Illumina测序。我们用甲状腺激素和MPI(以阻断TH的产生)来治疗黄曲霉，并将样本提交给转录分析。我们已经在黄曲霉中建立了CRISPR/Cas9技术，并针对第一个基因(Dio2)。

How differences in pigmentation arose have never been fully explained. In anemonefishes there is a difference in white bar formation between Amphiprion ocellaris and A. percula. As white bars are linked to thyroid hormones (TH) we study if this ontogeny difference is linked to differences in TH signaling. We collected samples of the 2 species from hatching until 100 days. We have added the closely related A. biaculeatus to determine the ancestral status of bar formation. The samples are ready and will soon be sent for Illumina sequencing. We treated A. ocellaris with thyroid hormone and to MPI (to block TH production) and we submitted the samples to transcriptomic analysis. We have established the CRISPR/Cas9 technology in A. ocellaris and have targeted a first gene (dio2).