High throughput microbial microculture and single cell analysis facility

高通量微生物微培养和单细胞分析设施

• 批准号: LE160100185
• 负责人: A/Prof Victoria Haritos
• 金额: $ 26.67万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Infrastructure, Equipment and Facilities
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-03-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LE160100185
• 关键词: throughput; microbial; microculture; single; cell

High throughput microbial microculture and single cell analysis facility: To support the emerging research area of microbial heterogeneity and variation in response to conditions, this project aims to establish a facility centred on a 24-microbioreactor system for high throughput microbial culturing. This is designed to be connected to two complementary analysis techniques – flow cytometry and high resolution infra-red microscopy – for the non-destructive measurement of metabolic activities and mapping of constituents of whole cells. This would help us to determine the variation in response between organisms, to guide cell line development and process optimisation for a wide range of biotechnology applications. Expected outcomes may apply to Australia’s brewing, wine, food processing, aquaculture, biofuels, biomedical and biotechnology industries.

高通量微生物微量培养和单细胞分析设施： 为了支持新兴的微生物异质性和条件变化的研究领域，该项目旨在建立一个以24-微型生物反应器系统为中心的高通量微生物培养设施。这是为了连接到两个互补的分析技术-流式细胞术和高分辨率红外显微镜-用于非破坏性测量的代谢活动和整个细胞的成分绘图。这将有助于我们确定生物体之间的反应变化，指导细胞系开发和广泛的生物技术应用的工艺优化。预期成果可能适用于澳大利亚的酿酒、葡萄酒、食品加工、水产养殖、生物燃料、生物医学和生物技术行业。