Neutrophil-Specific PET Cellular Imaging for ARDS

ARDS 中性粒细胞特异性 PET 细胞成像

• 批准号: 22H03027
• 负责人: 樋口 隆弘
• 金额: $ 11.07万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22H03027/
• 关键词: neutrophil; ARDS; CXCR2; PET imaging; PET; COVID-19

The goal of this study is to develop a PET imaging assay that can specifically detect neutrophils of the lung and other organs by targeting the CXCR2, achemokine receptor specifically expressed on neutrophils.At present, there is no in vivo imaging technique that can specifically visualize local neutrophil infiltration identified via systemic scanning of human body as well as animals. Therefore, we aim to achieve this by using cutting edge PET molecular imaging technology, which can visualize biomarker molecules with high sensitivity.Considering future clinical applications, to develop of small molecule tracers for F18-labeling that are easy to handle in clinical settings, we designed and synthesized PET tracer candidates targeting CXCR2 in this year. To evaluate the affinity of these radiotracers, cold references, i.e. their non-radioactive references, were synthesized first. The direct radiofluorination without the special preparation of tosylate precursor was also methodology investigation to facilitate the radiolabelling protocol from original structures. In contract, the cold references of urea series of compounds and the corresponding precursors for radiolabelling were also synthesized.

本研究的目标是开发一种PET成像检测方法，通过靶向中性粒细胞特异性表达的趋化因子受体CXCR2，可以特异性检测肺部和其他器官的中性粒细胞。目前，还没有体内成像技术可以特异性地可视化通过人体和动物的系统扫描识别的局部中性粒细胞浸润。因此，我们的目标是通过使用尖端的PET分子成像技术来实现这一目标，该技术可以高灵敏度地可视化生物标志物分子。考虑到未来的临床应用，为了开发易于临床操作的F18标记的小分子示踪剂，我们今年设计并合成了针对CXCR2的候选PET示踪剂。为了评估这些放射性示踪剂的亲和力，首先合成冷参考，即它们的非放射性参考。无需特殊制备甲苯磺酸盐前体的直接放射性氟化也是方法学研究，以促进原始结构的放射性标记方案。与此同时，还合成了尿素系列化合物的冷参考品和相应的放射性标记前体。

项目成果

Augsburg大学/Wuerzburg大学(ドイツ)

奥格斯堡大学/维尔茨堡大学（德国）

Imaging of C-X-C Motif Chemokine Receptor 4 Expression in 690 Patients with Solid or Hematologic Neoplasms Using 68Ga-Pentixafor PET

• DOI: 10.2967/jnumed.121.263693
• 发表时间: 2022-11-01
• 期刊: JOURNAL OF NUCLEAR MEDICINE
• 影响因子: 9.3
• 作者: Buck, Andreas K.;Haug, Alexander;Werner, Rudolf A.
• 通讯作者: Werner, Rudolf A.

18F-Labeled PET Imaging Probes Targeting Angiotensin II Type 1 Receptors

针对血管紧张素 II 1 型受体的 18F 标记 PET 成像探针

• 发表时间: 2022
• 作者: Xinyu Chen;Matthias Hoffmann;Rudolf A. Werner;Saskia Muehlig;Yusuke Yagi;Hiroyuki Kimura;Constantin Lapa;Michael Decker;Takahiro Higuchi
• 通讯作者: Takahiro Higuchi

C-X-C Motif Chemokine Receptor 4-Targeted Radioligand Therapy in Patients with Advanced T-Cell Lymphoma

• DOI: 10.2967/jnumed.122.264207
• 发表时间: 2023-01-01
• 期刊: JOURNAL OF NUCLEAR MEDICINE
• 影响因子: 9.3
• 作者: Buck, Andreas K.;Grigoleit, Goetz Ulrich;Werner, Rudolf A.
• 通讯作者: Werner, Rudolf A.