Intracellular organization of Ebola virus RNA synthesis; formation, maturation and transport of viral RNP (A05*)

埃博拉病毒RNA合成的细胞内组织；

• 批准号: 466756942
• 金额: --
• 依托单位: Institut für Virologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/466756942?language=en
• 关键词: Intracellular; organization; Ebola; virus; RNA

Replication of Ebola virus genomic RNA takes place in virus-induced inclusion bodies and results in the formation of progeny genomes that are packaged and transported to the sites of viral budding. Although virus-induced inclusion bodies play an important role in the synthesis of the genomic RNA, their organization is not well understood. In the proposed project we will investigate the spatial and temporal organization of Ebola virus RNA synthesis, its packaging into ribonucleoproteins (RNP), the molecular basis for RNP maturation to become transport-competent nucleocapsids, and cellular and viral factors involved in the transport.

埃博拉病毒基因组RNA的复制发生在病毒诱导的包涵体中，并导致子代基因组的形成，这些子代基因组被包装并运输到病毒出芽的位点。虽然病毒诱导的包涵体在基因组RNA的合成中起重要作用，但其组织结构尚不清楚。在拟议的项目中，我们将研究埃博拉病毒RNA合成的空间和时间组织，其包装成核糖核蛋白（RNP），RNP成熟成为运输能力核衣壳的分子基础，以及参与运输的细胞和病毒因子。