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Identification of genes regulating anthocyaninpigmentation in flower tepals of Asiatic hybrid lily.

亚洲杂交百合花被片花青素色素调节基因的鉴定。

基本信息

批准号：
14560022
负责人：
NAKATSUKA Akira
金额：
$ 1.73万
依托单位：
Shimane Universitiy
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

cDNA clones of three chalcone synthase(CHS) genes (LhCHSA,LhCHSB and LhCHSC) and one dihydroflavonol 4-reductase(DFR) gene (LhDFR) were isolated, and spatial and temporal expression of these genes was analyzed for two Asiatic hybrid lily cultivars, ‘Montreux' (pink tepals with spots) and ‘Connecticut King' (yellow tepals without spots). Pink tepals contain anthocyanin, and yellow tepals have no anthocyanin. In flower organs, three CHS genes were expressed in anthocyanin-pigmented tepals, filaments and pistils of ‘Montreux', and in unpigmented filaments and pistils of ‘Connecticut King'. Pigmented anthers accumulated only LhCHSC mRNA in both cultivars. LhDFR was abundantly expressed in pigmented tepals, anthers, filaments and pistils of ‘Montreux', and in pigmented anthers of ‘Connecticut King'.To clarify the genetic basis for carotenoid pigmentation in flower tepals of Asiatic hybrid lily, we evaluated the segregation of a tepal-carotenoid content among F_1 plants derived from a cross between ‘Montreux' (having a small amount of carotenoids) and ‘Connecticut King' (having a large amount of carotenoids), and mapped genetic loci for the carotenoid pigmentation onto the molecular linkage maps of ‘Montreux' and ‘Connecticut King' that we constructed previously. The tepal-carotenoid content among the F_1 plants showed continuous segregation, indicating that several genes are associated with this trait. Quantitative trait loci(QTL) analysis identified one QTL, qCARmon6, on the sixth linkage group of the ‘Montreux' map.qCARmon6 explained 58.2% of the total phenotypic variation, that is, this locus had a large effect on the carotenoid accumulation.

分离了三个查尔酮合酶（CHS）基因（LhCHSA、LhCHSB和LhCHSC）和一个二氢黄酮醇4-还原酶（DFR）基因（LhDFR）的cDNA克隆，并分析了两个亚洲杂交百合品种“蒙特勒”（带斑点的粉红色花被）和“康涅狄格”的这些基因的时空表达。 King'（黄色花被片，无斑点）。粉色花被片含有花青素，黄色花被片不含花青素。在花器官中，三个 CHS 基因在‘蒙特勒’的花青素色素花被片、花丝和雌蕊以及‘康涅狄格王’的未色素花丝和雌蕊中表达。两个品种的有色花药仅积累 LhCHSC mRNA。 LhDFR 在“蒙特勒”的有色花被片、花药、花丝和雌蕊以及“康涅狄格王”的有色花药中大量表达。为了阐明亚洲杂交百合花被片色素沉着的类胡萝卜素色素沉着的遗传基础，我们评估了来自亚洲杂交百合的 F_1 植物中花被片类胡萝卜素含量的分离。 ‘蒙特勒’（含有少量类胡萝卜素）和‘康涅狄格国王’（含有大量类胡萝卜素），并将类胡萝卜素色素沉着的遗传位点映射到我们之前构建的‘蒙特勒’和‘康涅狄格国王’的分子连锁图上。 F_1 植株中的花被类胡萝卜素含量表现出连续分离，表明多个基因与该性状相关。数量性状基因座（QTL）分析在‘蒙特勒’图谱第六连锁群上鉴定出1个QTL，qCARmon6。qCARmon6解释了总表型变异的58.2%，即该位点对类胡萝卜素积累有较大影响。