high resolution GC-EI-MSMS instrument

高分辨率GC-EI-MSMS仪器

• 批准号: 467386044
• 金额: --
• 依托单位: Universität Potsdam
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/467386044?language=en
• 关键词: resolution; GC; EI; MSMS; instrument

Mass spectrometry is a cross-sectional technology that plays a key role in chemical, biochemical and molecular biological research and teaching. Representatives of the Institute of Chemistry and the Institute of Biochemistry and Biology of the University of Potsdam have joined forces and combined the urgently needed replacements and a new acquisition of individual mass spectrometry instruments with the establishment of a cross-institute MS analytics center. Our aim is to strengthen synergy effects and to share and expand analytical expertise at the University of Potsdam. Further, we intend to coordinate the use of various analytical techniques, to enhance the overall capacity and to prevent repair bottlenecks. With main use in the Institute of Chemistry, here, we apply for a GC-EI-MSMS instrument for the analysis of small, easily vaporizable molecules to replace the old GC-EI mass spectrometer, which is prone to repair. The instrument will be used to perform high-resolution EI/CI measurements to determine accurate masses. The new possibility of recording MS/MS spectra for unambiguous characterization of the analytes should greatly expand the range of applications for specific research work in the field of tandem mass spectrometry. As part of the package proposal, the following additional equipment is also requested: 1) an LC-ESI-IMS-MSMS instrument for dissolved samples (replacement proposal and expansion to include the previously missing ion mobility spectrometry/Institute of Chemistry), 2) a MALDI-MSMS instrument into which samples are applied in crystalline matrix (replacement proposal/Institute of Biochemistry and Biology), and 3) an ultra-high resolution LC-ESI-Orbitrap-MSMS instrument for dissolved samples.Numerous groups from both institutes will benefit from higher performance and versatility of the instruments (including IMS and ultra-high resolution) as well as the significantly increased sample capacity and will be able to advance internationally competitive research projects.

质谱学是一门横截面技术，在化学、生物化学和分子生物学的研究和教学中发挥着关键作用。波茨坦大学化学研究所和生物化学与生物研究所的代表联合起来，将迫切需要的替代品和新获得的单个质谱仪与建立一个跨研究所的MS分析中心结合起来。我们的目标是加强协同效应，并分享和扩大波茨坦大学的分析专业知识。此外，我们打算协调各种分析技术的使用，以提高整体能力并防止修复瓶颈。主要用于化学研究所，在这里，我们申请了一台GC-EI-MSMS仪器，用于分析易蒸发的小分子，以取代旧的GC-EI质谱仪，这是容易维修的。该仪器将用于进行高分辨率的EI/CI测量，以确定准确的质量。记录MS/MS光谱以明确表征分析物的新可能性将极大地扩大串联质谱学领域中具体研究工作的应用范围。作为一揽子建议的一部分，还要求提供以下额外设备：1)用于溶解样品的LC-ESI-IMS-MSMS仪器(替换建议和扩展，以包括以前缺失的离子迁移谱/化学研究所)，2)MALDI-MSMS仪器，其中样品以晶体基质进行应用(替换建议/生物化学和生物研究所)，3)用于溶解样品的超高分辨率LC-ESI-Orbitrap-MSMS仪器。来自两个研究所的众多团队将受益于该仪器更高的性能和通用性(包括IMS和超高分辨率)以及显著增加的样品容量，并将能够推进具有国际竞争力的研究项目。