Premier GC/Q-TOF with headspace and SPME analysis

具有顶空和 SPME 分析功能的 Premier GC/Q-TOF

• 批准号: 529034606
• 金额: --
• 依托单位: Universität Münster
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/529034606?language=en
• 关键词: Premier; GC; TOF; headspace; SPME

The main research topic of the Ischebeck lab is the study of lipid droplets, subcellular structures that contain hydrophobic compounds and proteins embedded in the surrounding phospholipid monolayer. The study of the role of lipid droplets in metabolism (especially sterol metabolism), and the biochemical analysis of lipid droplet-associated enzymes of unknown function requires sensitive analytical tools. Biotechnological approaches to turn lipid droplets into factories and storage depots for terpenoids also needs possibilities to measure precursors and products. Furthermore, the Ischebeck lab has a strong track record of collaborative projects, where it provided gas chromatography-mass spectrometry-based analytics to groups of the plant, microbiology and neuroscience field. The recent transfer of the Ischebeck lab to the WWU Münster also sparked interest in these analytics for several projects at the faculty. The Ischebeck lab collected a lot of experience in GC-MS analytics but since the transfer of the group to the WWU, no state-of-the art gas chromatograph-mass spectrometer system is available at the institute or the location. However, a direct access to such an instrument is required for the projects of the lab and the collaborative efforts. The gas chromatograph-quadrupole-time of flight-mass spectrometer would provide highly sensitive analytics of hydrophobic substances both in targeted and untargeted approaches. The exact determination of the molecular mass of metabolites and their fragments would help to identify substances. By the addition of the headspace and solid-phase-microextraction modules, analysis of volatiles is strongly enhanced. Through derivatization, it is also possible to identify and absolutely quantify polar substances including sugars, amino acids, and other central metabolites. In conclusion, the instrument would provide highly sensitive and quantitative measurements of a large variety of compounds, strongly increasing the research possibilities of the lab and the faculty of biology.

Ischebeck实验室的主要研究课题是研究脂滴、含有疏水化合物的亚细胞结构以及嵌入周围磷脂单分子层的蛋白质。研究脂滴在新陈代谢(特别是固醇代谢)中的作用，以及对未知功能的脂滴相关酶进行生化分析，需要灵敏的分析工具。将脂滴转化为工厂和萜类化合物储存库的生物技术方法也需要有可能测量前体和产品。此外，Ischebeck实验室在合作项目方面有着良好的记录，在这些项目中，它为植物、微生物学和神经科学领域的小组提供基于气相色谱-质谱仪的分析。Ischebeck实验室最近转移到WWU明斯特，也引发了教职员工对这些分析项目的兴趣。Ischebeck实验室收集了许多GC-MS分析方面的经验，但自从该小组转移到WWU以来，该研究所或现场没有最先进的气相色谱-质谱仪系统。然而，实验室的项目和合作努力需要直接接触到这样的仪器。气相色谱-四极杆飞行时间-质谱仪将以定向和非定向的方法对疏水物质进行高度灵敏的分析。准确测定代谢物及其碎片的分子质量将有助于识别物质。通过添加顶空和固相微萃取模块，大大加强了挥发性物质的分析。通过衍生化，还可以识别和绝对定量包括糖、氨基酸和其他中心代谢物在内的极性物质。总之，该仪器将提供对多种化合物的高灵敏度和定量测量，极大地增加了实验室和生物学学院的研究可能性。