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Search for oviposition deterrents as novel pest-control agents

寻找产卵威慑剂作为新型害虫防治剂

基本信息

批准号：
14560039
负责人：
HONDA Keiichi
金额：
$ 2.3万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

项目摘要

1.Oviposition responses and larval performance in Papilio polytes and P.protenorOviposition response of P.polytes to the foliage of nine rutaceous plants belonging to nine genera, their extracts, and fractions thereof and larval performance on these plants were examined to determine their acceptability to the butterfly and the presence of oviposition stimulants and/or deterrents. These plants were arrayed as follows in decreasing order of acceptability : Toddalia asiatica (Ta) = Citrus depressa (Cd) = Glycosmis citrifolia (Gc) = Fagara ailanthoides (Fa) > Phellodendron amurense (Pa) > Evodia meliifolia (Em) >> Orixa japonica (Oj) ≧ Murraya paniculata (Mp) = Melicope triphylla (Mt). Mt exerted moderate oviposition deterrency and strong larvicidal activity, while Mp was found to contain both potent oviposition and feeding deterrent(s). We have identified two oviposition stimulants, trans-4-hydroxy-N-methyl-L-proline and 2-C-methyl-D-erythronic acid, from Ta and an oviposition deterrent, … More trigonelline, from Mp. On the other hand, larvae of P.protenor did not grow well on any one of Ta, Mt, and Mp. In oviposition bioassays, potent deterrent activity was evoked by Mp and Pa, while weak deterrent activity, by Em and Mt.2.Deterrent effect of plant extracts and fractions on egg-laying and reproductionThree water-soluble fractions derived from the extracts of Mp, Mt, and Pa, trigonelline, caffeine, phellamurin, and an isobutanol fraction of Mt were tested for their deterrent activity in egg-laying or reproduction in P.polytes or greenhouse white fly, using potted plants of C.depressa to which these samples were applied. (1)The aq. fractions of Mp and Mt strongly deterred the egg-laying by P.polytes, while other samples except Pa and phellamurin only weakly deterred the egg-laying (leaf-application method). (2)All samples but the control were found to depress the reproduction of the white fly in about 4 weeks (leaf-application method). (3)Several samples including trigonelline hydrochloride depressed the reproduction of the white fly to a certain extent (soil- application method). However, further investigations on the method of application and the concentration of samples applied seem to be needed to put these new pest control tactics into practice. Less

1.研究了多凤蝶对9属9种芸香植物叶片及其提取物和组分的产卵反应和幼虫在这些植物上的产卵表现，以确定它们对蝴蝶的可接受性以及是否存在促产卵剂和/或阻产卵剂。这些植物按可接受度的递减顺序排列为：亚洲棕榈(Ta) =柑橘(Cd) =柑橘糖(Gc) = ailanthoides (Fa) >黄柏(Pa) >五叶草(Em) >>日本龙柏(Oj)≧paniculata (Mp) =三叶Melicope （Mt）。Mt具有中等的阻卵作用和较强的杀幼虫作用，而Mp具有较强的阻卵和阻食作用。我们从Ta中鉴定出了两种促排卵剂，反式-4-羟基- n -甲基- l-脯氨酸和2- c -甲基- d -赤四酸，从Mp中鉴定出了一种促排卵剂，更多的trigonelline。另一方面，在Ta、Mt和Mp上均不能很好地生长。在产卵生物测定中，Mp和Pa具有较强的阻生活性，Em和Mt.2具有较弱的阻生活性。植物提取物和馏分对产卵和繁殖的阻生作用从Mp、Mt和Pa提取物中提取的三种水溶性馏分、葫芦巴碱、咖啡因、小茴香苷和Mt的异丁醇馏分对多蝇和温室白蝇的产卵和繁殖的阻生作用进行了测试。(1) Mp和Mt的aqq组分对多裂裂卵的产卵有较强的抑制作用，而除Pa和phellamurin外的其他样品对多裂裂卵的产卵只有较弱的抑制作用（施叶法）。(2)除对照外，其余样品在4周左右的时间内均能抑制白蝇的繁殖。(3)盐酸葫芦巴碱等几种样品在一定程度上抑制了白蝇的繁殖（土施法）。然而，为了将这些新的害虫防治策略付诸实施，似乎需要对施用方法和施用样品浓度进行进一步的调查。少