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Identification of Xanthomonas oryzae pv. oryzae secetor proteins involved in interactions with plants

米黄单胞菌 pv. 的鉴定

基本信息

批准号：
14560043
负责人：
TSUGE Seiji
金额：
$ 1.28万
依托单位：
Kyoto Prefectural University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560043/
关键词：
Xanthomonas oryzae pv. oryzae pathogenicity secretory proteins type III secretion system type II secretion system hrp イネ分子生物学 type III分泌タンパク質 typeIII分泌機構

项目摘要

Like some gram-negative plant-pathogenic bacteria, Xanthomonas oryzae pv. oryzae possesses hrp genes, which encode components of the type III secretion system. Several reports suggest that virulence proteins against susceptible rice cultivars and resistance-inducing proteins against resistant rice cultivars and nonhost plants are secreted via the secretion system. Previously we constructed a medium to induce expression of X. oryzae pv. oryzae hrp genes, which are naturally induced only in plants. In this study, we identified three hrp dependent type III secretory proteins using the medium. Two of them, HrpE1 and HrpF, were essential for pathogenicity against susceptible rice cultivars and induction of resistant response in resistant rice cultivars and nonhost plants. The other protein, Hpal, was shown to be involved in aggressiveness of the bacteria to host, plants.A product of hrpXo is reported to be hrp regulatory protein, and to function as a transactivator for hrp genes and genes whose products are secreted through the type III secretion system. In this study we also showed that HrpXo regulates not only expression of hrp genes and type III secretory protein genes but also that of type II secretory proteins. And we identified one of them as a cystein protease homolog.

像一些革兰氏阴性植物致病菌一样，米黄单胞菌pv。oryzae具有hrp基因，该基因编码III型分泌系统的成分。一些报道表明，对易感水稻品种的毒力蛋白和对抗性水稻品种和非寄主植物的抗性诱导蛋白是通过分泌系统分泌的。之前我们构建了一种诱导X. oryzae pv表达的培养基。仅在植物中自然诱导的米类HRP基因。在这项研究中，我们使用培养基鉴定了三种hrp依赖性III型分泌蛋白。其中HrpE1和HrpF是对易感水稻品种的致病性以及诱导抗性水稻品种和非寄主植物产生抗性反应所必需的。另一种蛋白质，Hpal，被证明与细菌对宿主植物的侵略性有关。据报道，hrpXo的产物是hrp调节蛋白，作为hrp基因和其产物通过III型分泌系统分泌的基因的反激活子。在这项研究中，我们还发现HrpXo不仅调节hrp基因和III型分泌蛋白基因的表达，还调节II型分泌蛋白的表达。我们确定其中一个是半胱氨酸蛋白酶同源物。