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Contribution assessment of hatchery origin black sea bream Acanthopagrus schlegeli into natural populations using microsatellite markers

利用微卫星标记评估孵化场黑鲷对自然种群的贡献

基本信息

批准号：
14560152
负责人：
UMINO Tetsuya
金额：
$ 2.62万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

项目摘要

Genetic diversity of hatchery origin black sea bream (Acanthopagrus schlegeli) released at Daio Bay in Japan was monitored during two consecutive years using 6 microsatellite DNA markers. The number of alleles per locus ranged from 6 to 20 while Ho averaged 0.83-0.88 at all loci. Departures from HWE were observed in all samples, showing heterozygosity excess in both offspring groups and deficit in the broodstock and wild samples. The 33.7% and 38.5% of the specimens in 2003 and 2004, respectively ; were identified as hatchery origin, with another 40% of the samples matching with a single parent each year, suggesting admixture with the natural population. The offspring groups presented a reduction of 28.9% of the alleles regarding the broodstock for the two years analyzed. The homogeneity between groups was also confirmed by the low values of Fst, warning about the necessity of monitoring the genetic diversity of black sea bream inhabiting Daio Bay during longer periods in order to avoid possible genetic drifts. The results obtained in the present study represent a clear example of how despite the high survival rates of previously released juveniles ; mating small number of breeders may unleash negative impact on genetic diversity. This experience evidences the necessity of estimating and monitoring the genetic evolution on natural resources whenever stock enhancement practices are carried out.

使用 6 个微卫星 DNA 标记连续两年监测了在日本大王湾放生的孵化场黑鲷 (Acanthopagrus schlegeli) 的遗传多样性。每个位点的等位基因数量范围为 6 至 20，而 Ho 在所有位点的等位基因数量平均为 0.83-0.88。在所有样本中都观察到与 HWE 的偏离，表明两个后代组中杂合性过剩，而亲鱼和野生样本中杂合性不足。 2003年和2004年的样本分别为33.7%和38.5%；被确定为孵化场来源，每年还有 40% 的样本与单亲匹配，表明与自然种群混合。在分析的两年中，后代群体的亲鱼等位基因减少了 28.9%。 Fst的低值也证实了群体之间的同质性，警告有必要在较长时期内监测栖息在Daio湾的黑鲷的遗传多样性，以避免可能的遗传漂移。本研究获得的结果清楚地表明，尽管先前释放的青少年的存活率很高；少量繁殖者交配可能会对遗传多样性产生负面影响。这一经验证明，每当进行种群增殖实践时，就有必要估计和监测自然资源的遗传进化。