Evaluation for stock efficiency of fish and shellfish in Hiroshima Bay by DMA marker

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Four highly variable microsatellite were isolated and then used to investigate genetic diversity and population structure in black sea bream, Acanthopagrus schlegeli, collected from wild population of six locations (western Japan and southern Korea), and hatchery stock from the Hiroshima Bay. There were no differences in genetic diversity between western Japan and hatchery-stock populations. According to the Nei's genetic distance, there was no geographically separated population in western Japan populations.We surveyed the genetic variability and pedigree tracing of stocked black sea bream produced from 51 breeders with 7 microsatellite loci for two years, 2000 and 2001. While the mean observed heterozygosity was not changed from broodstock to offspring, the mean number of alleles per locus decreased more than 16.5%. For 69.3% (69.7% in 2000 and 69.0% in 2001) of the offspring, one parental couple was determined using pedigree analysis. The actual contribution rate was 51.7% of females and 77.3% of males in 2000 and 48.3% of females and 72.7% of males in 2001. The evidence that contribution rate of female and male was 69.0% and 90.9% in totaling two years, respectively suggests more than 30% of females did not participate in either year. Using the number of actually participating contributor and correcting for unbalanced family size per parent, the effective number of contributors (Ne*) was40. When stock separation study conducted using 7 microsatellite loci after release, separation eddiciency was almost identical to ALC-tagging in Otolith. The microsatellite loci showed strong possibilities as genetic markers of black sea bream for genetic variability and divergence. We isolated microsatellite as a DNA marker in sea cucumber and rock fish.