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Genetic elucidation on mitochondrial DNAs regulating expression of economic traits of domestic animals

线粒体DNA调控家畜经济性状表达的遗传阐明

基本信息

批准号：
14560238
负责人：
NOMURA Koh
金额：
$ 2.11万
依托单位：
Tokyo University of Agriculture
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

项目摘要

The domestic livestock belonging to Bobidae have various economic traits. We have been trying to detect the specific variants in mitochondrial DNA sequences in Bobidae livestock that have some attractive economic traits such as heat tolerance, disease resistance, adaptation for highland, daily trait etc. We sequenced the complete mitochondrial genome of various Bobidae animals and compared between them. 1. Zebu cattle have some advantages over taurin cattle in abilities of heat tolerance and disease resistance. The complete mtDNAs of taurin and zebu cattle were 16,338bp. The amino acid sequences were conserved for ND2, COI, COII, ND4 and LND6 but showed low homology for ATPase8, ATPase6, ND5 and ND3. The amino acid substitutions of these regions showed the range between 0.9 and 3.1%. 2. We compared the complete mtDNA sequences between river and swamp buffaloes. River buffaloes have been specialized in daily breed, while swamp buffaloes have been used as draft animal. The complete mtDNA … More s of river and swamp buffaloes were 16,357bp and 16,355bp, respectively. The differences in length between river and swamp buffaloes are caused by variations in the D-loop region. The homologies were low for ND4L and ND5, while high for ATPase8, COI, COII ND3 and ND6. 3. Yak has advantage for adaptation to highland. Fourteen substitution of amino acid were specific to yaks. Out of eight shifted from hydrophilic to hydrophobic. Ten DNA substitutions were observed in tRNA regions. Out of three were concentrated in tRNA-phenylalanine region. 4. To analyze the effect of domestication, we detected the variant region between domestic goats and Bezore, that is considered the ancestor of domestic goat. The domestic goats were classified into four types on the D-loop sequences. The sequences were conserved for ND1, ND2, COI, D4 and ND6 among the types. The value of amino acid substitutions of ATPase8, ATPase6. COII COIII, ND3 and ND4L showed the range between 0.4〜3.1%. The complete sequence of mtDNA of Bezore was identified with that of the most common type of domestic goats. Less

博比科家畜具有多种经济性状。本研究对博比科动物线粒体基因组进行了全序列测定，并对博比科动物线粒体基因组进行了比较。1.与牛磺酸牛相比，瘤牛在耐热性和抗病性方面具有一定的优势。牛、瘤牛的mtDNA全长为16，338 bp。ND 2、COI、COII、ND 4和LND 6的氨基酸序列是保守的，但与ATPase 8、ATPase 6、ND 5和ND 3的同源性较低。这些区域的氨基酸取代显示在0.9和3.1%之间的范围。2.我们比较了河流水牛和沼泽水牛的mtDNA全序列。河水牛专门用于日常繁殖，而沼泽水牛则被用作驮畜。完整的mtDNA ...更多信息河流型水牛和沼泽型水牛的S分别为16，357 bp和16，355 bp。河流水牛和沼泽水牛之间的长度差异是由D环区域的变化造成的。ND 4 L和ND 5的同源性较低，而ATPase 8、COI、COII ND 3和ND 6的同源性较高。3.牦牛具有适应高原的优势。有14个氨基酸发生了牦牛特有的置换。八个从亲水性转变为疏水性。在tRNA区域观察到10个DNA替换。三种主要集中在tRNA-苯丙氨酸区域。4.为了分析驯化的效果，我们检测了家山羊与被认为是家山羊祖先的Bezore之间的变异区域。根据D-loop序列将家山羊分为4种类型。ND 1、ND 2、COI、D4和ND 6的序列在各类型间比较保守。ATPase 8、ATPase 6的氨基酸取代值。COII、COIII、ND 3和ND 4L的范围在0.4 ~ 3.1%之间。Bezore的线粒体DNA的全序列与最常见的类型的国内山羊。少