Risk analysis of staphylococcal enterotoxin type G, H, I, and J in milk and meat.

牛奶和肉类中 G、H、I 和 J 型葡萄球菌肠毒素的风险分析。

• 批准号: 14560259
• 负责人: SHINAGAWA Kunihiro
• 金额: $ 2.5万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560259/
• 关键词: S.aureus; Staphylococcal enterotoxin (SE); recombinant DNA; enterotoxin gene (se-gene); enterotoxin production; Staphylococcal enterotoxin; emesis; diagnosis; SEG; SEH; SEI; S. aureus

In recent years, many new staphylococcal enterotoxins (SEs) have been reported. To investigate pathobiology and epidemiology of these newly identified SEs, we had undertaken studies shown in below.1. To establish immunological detection methods of newly identified SEs, we have developed E.coli expression system of SEs to obtain large amount of each purified SE. These purified SEs were immunized to rabbits, and we obtained monospecific antibodies to each SEs.2. We identified and characterized a novel staphylococcal entertoxin (SE)-like putative toxin, named SER. Nucleotides sequencing analysis of ser gene revealed that ser was most closely related to seg gene. The ser gene product, SER, was successfully expressed as recombinant protein in an Escherichia coli expression system, and rSER showed significant T cell stimulation activity. MHC class II molecules were required for T cell stimulation by SER. SER stimulated T cells bearing receptors Vβ 3, 11, 12, 13.2, and 14. These results sugge … More sted that SER acts as a superantigen. The SER production in ser harboring S.aureus strains was confirmed by Western blot analysis using anti-rSER antibody. Moreover, ser was seen to be encoded by at least two types of plasmids. In particular, one kind of plasmid encoding the ser gene has been known as sed-and sej-encoding pIB485-related plasmid (Infection and Immunity 71:6088, 2003 ; Infection and Immunity 72:3664, 2004).3. To investigate the distribution of staphylococcal enterotoxin (SE) A to R (SEA to SER) genes (sea to ser) in Staphylococcus aureus, we have developed multiplex PCR for detection of 17 kinds of SE genes (sea to see, seg to selr) and TSST-1 gene. Using this multiplex PCR system, we investigated SE-genotype of Staphylococcus aureus 155 isolates obtained from bovine raw milk in Japan. In almost strains, these SE-genotypes were consistent with known SEs gene profile of Genomic islands or plasmids. These results suggest that SE-genotype of most S.aureus would be determined by combination of harboring mobile genetic elements. (manuscript in preparation).4. We have investigated growth and enterotoxin (SEA) production of S.aureus in different concentrated skim milk (15, 25, 35%) at different storage (15, 25, 35℃). The growth rate of S.aureus was more rapid at 35℃ than 15 and 25℃ and SEA was produced most highly at 35℃ storage. Less

近年来，许多新的葡萄球菌肠毒素（SE）被报道。为了研究这些新发现的SE的病理生物学和流行病学，我们进行了以下研究。为了建立新鉴定SE的免疫学检测方法，我们建立了SE的大肠杆菌表达系统，以获得大量纯化的SE。将纯化后的SE免疫家兔，获得了针对各SE的单特异性抗体.我们鉴定并鉴定了一种新的葡萄球菌肠毒素（SE）样推定毒素（SER），对ser基因的核苷酸序列分析表明，ser与seg基因的同源性最高。在大肠杆菌表达系统中成功地表达了ser基因产物SER，并显示出显著的T细胞刺激活性。SER刺激的T细胞携带受体Vβ 3、11、12、13.2和14。这些结果表明， ...更多信息 认为SER是一种超抗原。通过使用抗rSER抗体的蛋白质印迹分析证实了携带SER的金黄色葡萄球菌菌株中的SER产生。此外，ser被认为是由至少两种类型的质粒编码。特别地，编码ser基因的一种质粒已知为编码sed和sej的pIB 485相关质粒（Infection and Immunity 71：6088，2003 ; Infection and Immunity 72：3664，2004）。为了解金黄色葡萄球菌肠毒素（SE）A to R（SEA to SER）基因（sea to ser）在金黄色葡萄球菌中的分布，建立了多重PCR检测17种SE基因（sea to see，seg to selr）和TSST-1基因的方法。使用这种多重PCR系统，我们调查了从日本牛原料奶中获得的金黄色葡萄球菌155株的SE基因型。在大多数菌株中，这些SE基因型与已知的基因组岛或质粒的SE基因图谱一致。这些结果表明，大多数金黄色葡萄球菌的SE基因型可能是由携带移动的遗传元件的组合决定的。（手稿正在编写中）。研究了金黄色葡萄球菌在不同浓度脱脂乳（15、25、35%）中的生长和产肠毒素（SEA）情况。金黄色葡萄球菌在35℃下生长速度快于15 ℃和25℃，SEA在35℃下产率最高。少

项目成果

Omoe K et al.: "Identification and characterization of a new staphylococcal enterotoxin-related putative toxin encoded by two kinds of plasmids."Infection and Immunity. 71(10). 6088-6094 (2003)

Omoe K 等人：“由两种质粒编码的新型葡萄球菌肠毒素相关推定毒素的鉴定和表征。”感染和免疫。

Hu, D.-L., Omoe, K., Sasaki, S., Sashinami, H., Sakuraba, H., Yokomizo, Y., Shinagawa, K., Nakane, A.: "Vaccination with non-toxic mutant TSST-1 protects Staphylococcus aureus infection."J.Infect.Dis.. 188(3). 743-752 (2003)

Hu，D.-L.，Omoe，K.，Sasaki，S.，Sashinami，H.，Sakuraba，H.，Yokomizo，Y.，品川，K.，Nakane，A.：“用无毒突变体进行疫苗接种

Omoe, K., Imanishi, K., Hu, D.-L., Kato, H., Takahashi-Omoe, H., Nakane, A., Uchiyama, T, Shinagawa, K.: "Biological properties of staphylococcal enterotoxin-like toxin type R."Infect.Immun.. 72(6). 3664-3667 (2004)

Omoe, K.、Imanishi, K.、Hu, D.-L.、Kato, H.、Takahashi-Omoe, H.、Nakane, A.、Uchiyama, T、品川, K.：“葡萄球菌肠毒素的生物学特性

Hu, D.-L, Omoe, K., Shimoda, Y., Nakane, A., Shinagawa, K.: "Induction of emctic response of staphylococcal enterotoxins in the house musk shrew (Suncus murinus)"Infection and Immunity. 71. 567-570 (2003)

Hu，D.-L，Omoe，K.，Shimoda，Y.，Nakane，A.，品川，K.：“在家麝鼩（Suncus murinus）中诱导葡萄球菌肠毒素的催吐反应”感染和免疫。

Omoe, K.et al.: "Identification and characterization of a new staphylococcal enterotoxin-rerated putative toxin encoded by two kinds of plasmids"Infection and Immunity. 71(10). 6088-6094 (2003)

Omoe, K.等人：“由两种质粒编码的新型葡萄球菌肠毒素相关推定毒素的鉴定和表征”感染和免疫。