Studies on Structure and Fanction of Staphylococcal Enterotoxin and Bacillus cereus emetic toxin

葡萄球菌肠毒素和蜡样芽孢杆菌催吐毒素的结构与作用研究

• 批准号: 07660414
• 负责人: SHINAGAWA Kunihiro
• 金额: $ 1.41万
• 依托单位: Faculty of Agriculture, Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660414/
• 关键词: Staphylococcus aureus; Enterotoxin A; Synthetic peptide; Epitope; INF-; Bacillus cereus; HEp-2 vacuole factor; Emetic toxin; Suncus; 抗ペプチド抗体; エビトープ; セレウス菌; 嘔吐毒; エンテロトキシン; 催吐活性; 致死活性

The staphylococcal enterotoxins (SEs) are serologically classified in five types(A,B,C : C_1, C_2, C_3, D,and E). It was not yet fully understood by these studies to anaylyze the biologically and functionalyy active sites of SES.Attempts were made to investigate the antigenic determinants (epitopes) of SE-A.The results obtained were as follows.1.Native SE-A molecule contains at least 3 different antigenic determinants (synthetic peptides A-1 : amino acid residues 1-20, A-5 : 81-100 and A-8 : 141-160).2.Amino acid residue 161-180 on SE-A molecule is an essential region for production of murine INF-.To study the correlation between emetic toxin and HEp-2 vacuole activity produced by Bacillus cereus isolated from an outbreak of vomiting type food poisoning, emetic activities of purified HEp-2 factor (Cereulid) to rhesus monkeys and suncus were determined. The results obtained were as follows.1.The purified Cereulide (HEp-2 factor) is strongly suggested to be an emetic toxin itself.2.The Cereulide is stable in the digestive tracts, induce emesis, and show lethal activity leading to be cellular damage.

金黄色葡萄球菌肠毒素（SE）可分为A、B、C五型：C_1、C_2、C_3、D和E。这些研究还没有完全理解SES的生物学和功能活性位点。结果表明：1.天然SE-A分子至少含有3个不同的抗原决定簇（合成肽A-1：氨基酸残基1-20，A-5：81-100和A-8：2. SE-A分子上161-180位氨基酸残基是产生INF-的必需区域，2.从一起呕吐型食物中毒暴发中分离的蜡样芽孢杆菌（Bacillus cereus）产生空泡活性，测定了纯化的HEp-2因子（Cereulid）对恒河猴和梭子鱼的催吐活性。结果表明：1.纯化的思瑞利（HEp-2因子）本身是一种催吐毒素; 2.思瑞利在消化道内稳定，具有催吐作用，并具有致细胞损伤的致死活性。

项目成果

Shinagawa, K.et.al.: "Production and characterization of the vacuolation factor association with emetic activity of Bacillus cereus." Molecular Approaches to Food Safety Eklund,M.et.al.eds.319-328 (1995)

Shinakawa, K.et.al.：“与蜡状芽孢杆菌催吐活性相关的空泡因子的产生和表征。”

Hu,D-L.et.al.: "Identification of immunologically functional site (s) on staphylococcal enterotoxin A molecule associated with production of murine gamma interferon by using various synthetic peptides." Can.J.Microbiol.(submitted). (1998)

Hu,D-L.et.al.：“通过使用各种合成肽鉴定与鼠γ干扰素产生相关的葡萄球菌肠毒素 A 分子上的免疫功能位点。”

Hu, D-L.et.al.: "Epitope analysis of staphylococcal enterotoxin A using different synthetic peptides." J.Vet.Med.Sci.(submitted). (1998)

Hu, D-L.et.al.：“使用不同合成肽对葡萄球菌肠毒素 A 进行表位分析。”

胡 東良ら: "ブドウ球菌エンテロトキシンA分子上のIFN-γ産生誘導活性部位" 第125回日本獣医学会(宇都宮). (1997)

Dongliang Hu等人：“葡萄球菌肠毒素A分子上诱导IFN-γ产生的活性位点”第125届日本兽医协会（宇都宫）（1997年）。

上田 成子 ら: "大豆および豆腐におけるBascillus thuringiensisについて." 日本食品微生物学雑誌. 12. 249・255 (1996)

Shigeko Ueda 等人：“关于大豆和豆腐中的苏云金杆菌。”日本食品微生物学杂志 12. 249・255 (1996)