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Physiological roles of estrogen and TGF-α in the maintenance of pregnancy

雌激素和TGF-α在维持妊娠中的生理作用

基本信息

批准号：
14560269
负责人：
TAMADA Hiromichi
金额：
$ 1.98万
依托单位：
Osaka Prefecture University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560269/
关键词：
estrogen pregnancy uterus fetus aromatase inhibitor TGF-α EGF receptor

项目摘要

Pregnancy and its maintenance are essential for reproduction of farm animals. This study was performed to clarify the physiological roles of estrogen and TGF-α in pregnancy, and the final objective is to contribute towards the increase of pregnancy rates in farm animals.Continuous treatment with an inhibitor of estrogen. production, fadrozole hydrochloride (Fad), during early pregnancy delayed preimplantation embryo development, zona shedding by embryos and the period of maximum sensitivity to a decidualizing stimulus in rats. Since the treatment delayed the initiation of implantation by 1 or 2 days, it was speculated that estrogen might stimulate the embryo development and prepare the uterus for implantation. Continuous treatment with Fad during late pregnancy reduced the concentration of plasma estrogen and increased the uterine mRNA expression of lysyl oxidase (LO), which is necessary for the formation of intra-and inter-molecular cross-links of collagen. The treatment reduced the s … More ize and compliance of the uterine tissue framework and increased the intrauterine pressure by 4-fold to cause injuries in about one-quarter of fetuses. These results suggest that estrogen inhibits uterine expression of LO and keeps the physical environment suitable for the rapidly growing fetuses in late pregnancy. On the other hand, expression of TGF-α and its receptor (EGF-R) in the embryo and endometrium was found in the ovine implantation process, and it was speculated that TGF-α might stimulate the embryo growth and regulate proliferation and differentiation of endometrial cells. Furthermore, expression of TGF-α and EGF-R was observed in the canine endometrium, and the possibility of their involvement in the proliferation, differentiation and regression of endometrial epithelial cells was suggested.In conclusion, estrogen enhances the embryo growth and regulates the proliferation, difforentiation and regression of endometrial cells, resulting in establishment of implantation. Estrogen also regulates uterine expression of LO and keeps the uterine physical states suitable for fatal growth. Furthermore, TGF-α expressed in the uterus may play important roles in the embryo growth and the proliferation, differentiation and regression of endometrial cells. Less

妊娠及其维持对于家畜的繁殖至关重要。本研究旨在阐明雌激素和TGF-α在妊娠中的生理作用，最终目的是提高农场动物的妊娠率。持续使用雌激素抑制剂治疗。在大鼠妊娠早期，盐酸法倔唑（Fad）的产生延迟了植入前胚胎发育、胚胎脱落和对蜕膜刺激的最大敏感期。由于给药使着床开始延迟1或2天，因此推测雌激素可能刺激胚胎发育并使子宫准备着床。在妊娠晚期连续治疗Fad降低了血浆雌激素的浓度，并增加了赖氨酰氧化酶（LO），这是必要的胶原蛋白的分子内和分子间交联的形成的子宫mRNA的表达。治疗减少了s ...更多信息子宫组织框架的尺寸和顺应性，并使子宫内压力增加4倍，导致约四分之一的胎儿受伤。这些结果表明，雌激素抑制子宫LO的表达，并保持物理环境适合于快速生长的胎儿在妊娠后期。另一方面，在绵羊着床过程中，TGF-α及其受体（EGF-R）在胚胎和子宫内膜中均有表达，推测TGF-α可能促进胚胎生长，调节子宫内膜细胞的增殖和分化。结果表明，雌激素促进胚胎生长，调节子宫内膜细胞的增殖、分化和退化，从而促进着床的建立。雌激素还调节LO的子宫表达，并保持子宫的物理状态适合致命的增长。TGF-α在子宫中的表达可能对胚胎的生长发育及子宫内膜细胞的增殖、分化和退化起重要作用。少