ACTION AND ROLE OF EGF IN THE UTERUS

EGF 在子宫中的作用和作用

• 批准号: 10839013
• 负责人: TAMADA Hiromichi
• 金额: $ 2.18万
• 依托单位: OSAKA PREFECTURE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10839013/
• 关键词: HB-EGF; EGF; progesterone; implantation; pregnancy; uterus

Clarifying the mechanisms of implantation and maintenance of pregnancy could contribute to not only increase in efficiency of domestic and wild animal reproduction but also tracing human infertility to its origin and regulation of birth rates.Many growth factors are expressed in the embryo and pregnant uterus, and these factors could play important role in implantation and maintenance of pregnancy. This study clarified that heparin-binding EGF-like growth factor (HB-EGF) has actions to stimulate development of preimplantation embryo and to induce implantation in the rat. Furthermore, infusion of very small amounts of anti-HB-EGF antibody into the uterine lumen inhibited implantation thereafter. It was speculated from these results that HB-EGF plays important roles during the implantation process. In addition, this study showed that in the goat uterus EGF and EGF receptor are localized mainly is the luminal and glandular epithelium, and infusion of EGF into the uterine lumen inhibits co … More ntractility of the uterus. This result suggests a possibility that EGF family of growth factors may contribute to implantation and maintenance of pregnancy through the regulation of uterine contraction. By the way, treatments with progesterone (P) antagonists interrupt pregnancy, because P is essential for maintenance of pregnancy. This study showed that even under this deficiency of P actions the fetuses continue to grow by incising the uterus to release fetuses from uterine pressure in late pregnant rats. The result suggests that P contributes to the maintenance of pregnancy by making the uterine physical environment suitable for fetal survival and growth. As it has been reported that expressions of some EGF-family members in the uterus are stimulated by P, it is likely that parts of P actions on the uterus is mediated by the family members. This possibility remains to be elucidated.In conclusion, this study strongly supports the idea that the EGF family of growth factors plays important roles in the implantation process and maintenance of pregnancy. Less

阐明胚胎着床和妊娠维持的机制不仅有助于提高家畜和野生动物的繁殖效率，而且有助于人类不孕症的起源和生育率的调控。胚胎和妊娠子宫中表达多种生长因子，这些因子可能在胚胎着床和妊娠维持中发挥重要作用。本研究阐明了肝素结合EGF样生长因子（HB-EGF）具有促进大鼠植入前胚胎发育和诱导着床的作用。此外，输注非常少量的抗HB-EGF抗体到子宫腔抑制植入后。从这些结果推测，HB-EGF在植入过程中起重要作用。此外，本研究还表明，山羊子宫中EGF及其受体主要定位于子宫腔上皮和腺上皮，向子宫腔输注EGF可抑制EGF受体的表达。 ...更多信息 子宫的伸缩性这一结果表明，EGF家族的生长因子可能有助于通过调节子宫收缩着床和维持妊娠。顺便说一句，孕酮（P）拮抗剂治疗中断妊娠，因为P是维持妊娠所必需的。这项研究表明，即使在这种缺乏磷的行动，胎儿继续增长，通过切割子宫释放胎儿从子宫压力在怀孕后期的大鼠。结果表明，P有助于维持妊娠，使子宫的物理环境适合胎儿的生存和生长。已有研究表明，P刺激子宫中某些EGF家族成员的表达，因此P对子宫的部分作用可能是由家族成员介导的。总之，本研究强烈支持EGF家族生长因子在着床过程和妊娠维持中起重要作用的观点。少

项目成果

H.Tamada, C.Yoh, T.Inaba, H.Takano, N.Kawate, T.Sawada: "Epidermal growth factor (EGF) in the goat uterus : Immunohistochemical localization of EGF and EGF receptor and effect of EGF on uterine activity in vivo."Theriogenology. (in press).

H.Tamada、C.Yoh、T.Inaba、H.Takano、N.Kawate、T.Sawada：“山羊子宫中的表皮生长因子 (EGF)：EGF 和 EGF 受体的免疫组织化学定位以及 EGF 对子宫活动的影响

H.Tamada: "Expressions of growth factors in the uterus during implantation and their physiological roles."The Kansai Journal of Animal Science and Technology. 144 (in Japanese). 1-6 (2000)

H.Tamada：“着床过程中子宫内生长因子的表达及其生理作用。”关西动物科学技术杂志。

H. Tamada: "A progesterone antagonist cannot prevent fetal survival if the uterine horn is incised"Endocrine Journal. 45(6). 785-789 (1998)

H. Tamada：“如果子宫角被切开，黄体酮拮抗剂不能阻止胎儿存活”《内分泌杂志》。

玉田尋通: "着床期の子宮における成長因子の発現とその生理的役割に関する研究"関西畜産学会報. 144. 11-6 (2000)

Hiromichi Tamada：“着床期间子宫内生长因子的表达及其生理作用的研究”关西动物学会通报144. 11-6 (2000)。

H. Tamada: "Epidermal growth factor (EGF) in the goat uterus ; immunohistochemical localization of EGF and EGF receptor and effect of EGF on uterine activity in vivo"Theriogenology. (in press).

H. Tamada：“山羊子宫中的表皮生长因子（EGF）；EGF 和 EGF 受体的免疫组织化学定位以及 EGF 对体内子宫活动的影响”动物发生学。