Eucalyptus polyphenols inhibit intestinal fructose absorption, prevent adiposity, and improve diabetes mellitus

桉树多酚抑制肠道果糖吸收，预防肥胖，改善糖尿病

• 批准号: 14560283
• 负责人: MIYATAKE Kazutaka
• 金额: $ 2.11万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560283/
• 关键词: Metabolic Engineering; Citrate; Citrate transporter; Polyglutamic Acid; Bacillus natto; Gene Transportation; トランスポーター; 遺伝子操作; バチルス; 大腸菌; 機能発現; 大量生産

We investigated the citric acid transporter genes in Bacillus and cloned some of the genes. We expressed these genes in E.coli and measured the citrate transport activities. These transport activities were raised in special cultivation conditions, especially the aeration and magnesium concentration. Furthermore, we investigated the incorporation of citric acid through transporters in genes introduced into bacteria, Bacillus natto. We found the polyglutamic acid production was increased by the introduction of the citric acid transporter gene. We also found that the cultivation conditions, especially the oxygen density, the metal ions, especially magnesium, and the extract of yeast influenced the citric acid incorporation in the transformed bacteria.A 4-5 times productivity was obtained in comparison with the control production conditions.Introduction of a yraO gene that is thought as a citric acid transporter gene from the genomes of the bacillus, also increased the growth and incorporation of citric acid in medium.Incorporation of heavy metals in the bacterial cells were accelerated with the co-presence of citric acid, this suggests that this method is useful for heavy metal purification in an polluted environment, the so called bioremediation.

我们研究了芽孢杆菌中柠檬酸转运蛋白基因，并克隆了部分基因。我们在大肠杆菌中表达了这些基因，并测量了柠檬酸盐转运活性。在特定的培养条件下，特别是通气量和镁浓度下，这些转运活动增强。此外，我们研究了柠檬酸通过转运蛋白引入细菌，纳豆芽孢杆菌基因的掺入。我们发现柠檬酸转运蛋白基因的导入提高了聚谷氨酸的产量。我们还发现，培养条件，特别是氧气浓度，金属离子，特别是镁离子，和酵母提取物影响转化细菌中柠檬酸的掺入，与对照生产条件相比，获得了4-5倍的生产率。从芽孢杆菌基因组中导入被认为是柠檬酸转运蛋白基因的yraO基因，柠檬酸的存在促进了重金属在细菌细胞中的整合，这表明该方法可用于污染环境中重金属的净化，即所谓的生物修复。

项目成果

担子菌子実体抽出物に含まれる抗酸化成分

担子菌子实体提取物中含有的抗氧化成分

• 发表时间: 2005
• 期刊: 日本菌学会西日本支部会報 14
• 作者: 高井雄一郎;上田光宏;宮武和孝
• 通讯作者: 宮武和孝

共著: "食の安全(担当分)環境ホルモンの恐怖 141-172ページ"NTS. 244 (2003)

合著者：“食品安全（负责任）：对环境激素的恐惧，第 141-172 页”NTS 244 (2003)。

Comprehensive analysis of the effect of phytoestrogen, daidzein, on a testicular cell line, using mRNA and protein expression profile.

使用 mRNA 和蛋白质表达谱综合分析植物雌激素、大豆苷元对睾丸细胞系的影响。

• 发表时间: 2005
• 期刊: Food Chem.Toxicol. 43
• 作者: M.Isaji et al.;H.Wang et al.;N.Yamada et al.;H.Ueno et al.;H.Tanahashi et al.;Okuno Y;Adachi T;Ishida A;Hosoda C;Zhu S;Lazaro-Suarez ML;Kagaya S;Zacharia J;Sugimoto Y;Nagata N;Egashira N;Kagaya S;Chen Q;Erami C;Katsuma S;Adachi T;Katsuma S;Yamada M;Yamauchi J;Hosoda C;Adachi T
• 通讯作者: Adachi T

Family 19 Chitinase form Aeromonas sp.No.10S-24 : Role of Chitin-Bidong Domain in the Enzyme Activity

家族 19 几丁质酶形式气单胞菌 sp.No.10S-24：几丁质-Bidong 结构域在酶活性中的作用

• 发表时间: 2005
• 期刊: J.Biohem. 137
• 作者: Kojima;M.;Ueda;M.;Miyatake;K.;et al.
• 通讯作者: et al.

M.Inui, T.Adachi, M.Ueda, C.Mori, T.Iguchi, K.miyatake: "Effect of UV screens and preservatives on vitellogenin and choriogenin production in male madaka (Oryzias latipes)"Toxicology. 194. 43-50 (2003)

M.Inui、T.Adachi、M.Ueda、C.Mori、T.Iguchi、K.miyatake：“紫外线屏蔽和防腐剂对雄性 Madaka (Oryzias latipes) 中卵黄蛋白原和绒毛蛋白生成的影响”毒理学。