Eucalyptus polyphenols inhibit intestinal fructose absorption, prevent adiposity, and improve diabetes mellitus

研究了芽孢杆菌中柠檬酸转运体基因，并克隆了部分基因。我们在大肠杆菌中表达了这些基因，并测量了柠檬酸盐的转运活性。在特殊的栽培条件下，特别是曝气和镁浓度提高了这些转运活性。此外，我们研究了柠檬酸通过基因转运体导入细菌纳豆芽孢杆菌。我们发现柠檬酸转运基因的引入增加了聚谷氨酸的产量。我们还发现，培养条件，特别是氧密度，金属离子，特别是镁，酵母提取物影响转化菌中柠檬酸的掺入。与对照生产条件相比，生产效率提高了4-5倍。从芽孢杆菌基因组中引入一种被认为是柠檬酸转运基因的yraO基因，也增加了培养基中柠檬酸的生长和掺入。柠檬酸的存在加速了重金属在细菌细胞中的掺入，这表明该方法可用于污染环境中的重金属净化，即所谓的生物修复。

We investigated the citric acid transporter genes in Bacillus and cloned some of the genes. We expressed these genes in E.coli and measured the citrate transport activities. These transport activities were raised in special cultivation conditions, especially the aeration and magnesium concentration. Furthermore, we investigated the incorporation of citric acid through transporters in genes introduced into bacteria, Bacillus natto. We found the polyglutamic acid production was increased by the introduction of the citric acid transporter gene. We also found that the cultivation conditions, especially the oxygen density, the metal ions, especially magnesium, and the extract of yeast influenced the citric acid incorporation in the transformed bacteria.A 4-5 times productivity was obtained in comparison with the control production conditions.Introduction of a yraO gene that is thought as a citric acid transporter gene from the genomes of the bacillus, also increased the growth and incorporation of citric acid in medium.Incorporation of heavy metals in the bacterial cells were accelerated with the co-presence of citric acid, this suggests that this method is useful for heavy metal purification in an polluted environment, the so called bioremediation.