Analyzes of the diversity of secretory granules in the anterior pituitary endocrine cells.

垂体前叶内分泌细胞分泌颗粒多样性分析。

• 批准号: 14570001
• 负责人: SAKAI Yuko
• 金额: $ 1.92万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570001/
• 关键词: anterior pituitary; secretory granules; postnatal development; immunocytochemistry; chromogranins; secretogranins; gonadotropes; sexual maturation; プロラクチン産生細胞; 甲状腺刺激ホルモン産生細胞; 細胞分化

Five types of endocrine cells are known to be present in the anterior lobes of mammalian pituitary glands : corticotropes, gonadotropes, thyrotropes, mammotropes, somatotropes. Each endocrine cell possesses characteristic secretory granules and secretes distinct peptide hormones in response to appropriate stimuli. To elucidate the diversity of the secretory granules in this tissue, we first investigated the immunocytochemical localization of granins in the rat anterior pituitary in detail. As results, the localization pattern of SgIII in the anterior pituitary gland was similar to that of CgB at both light and electron microscopic levels, suggesting that the expressions of these two granins are regulated by common mechanisms. Moreover, we observed SgIII-positive intermediate endocrine cells that expressed both PRL and TSH, probably common precursor-like cells of these two endocrine cells. These findings suggest that the pituitary-specific transcriptional factors could regulate both the … More fate of pituitary stem cells and the expression of granins, which might contribute to the establishment of characteristic ultrastructures and the sizes of secretory granules in the pituitary endocrine cells. Based on these findings, we further examined changes in the expression level of granins and ultrastructure of pituitary gonadotropes during postnatal development of rats. Until 3 weeks after birth, no significant sex-related differences were found in the ultrastructure of pituitary gonadotropes ; gonadotropes of both sexes at birth possessed only a few small-sized secretory granules, and gradually large-sized secretory granules containing CgA appeared in these cells. However, the difference in pituitary gonadotropes between male and female rats became prominent since 3 weeks after birth ; large-sized granules containing CgA disappeared from female gonadotropes after this time point, whereas those granules were retained in male gonadotropes. These findings suggest that the expression of CgA is differently regulated by sex steroids after the beginning of sexual maturation, resulting in the sex-related differences in the composition and ultrastructure of secretory granules.Summing up, these findings suggest that the expression levels of granins affect the ultrastructure and size of secretory granules. Further investigations on the regulatory mechanisms of each granin expression would be required to clarify the diversity of secretory granules in the pituitary endocrine cells. Less

已知五种类型的内分泌细胞存在于哺乳动物垂体的前叶中：皮质动物，促性腺激素，甲状腺激素，乳房乳腺激素，乳腺体机体，躯体机体。每个内分泌细胞都具有特征性的秘密颗粒和秘密，以应对适当的刺激。为了阐明该组织中秘密颗粒的多样性，我们首先研究了详细介绍大鼠前垂体中格拉氏素的免疫细胞化学定位。结果，垂体前腺体中SGIII的定位模式与在光和电子显微镜水平上都类似于CGB的定位模式，这表明这两个花格兰蛋白的表达受到共同机制的调节。此外，我们观察到SGIII阳性的中间内分泌细胞，它们同时表达了这两个内分泌细胞的PRL和TSH，可能是常见的前体样细胞。这些发现表明，垂体特异性的转录因子可以调节……垂体干细胞的更多命运和花岗岩的表达，这可能有助于建立特征性的超微结构和垂体内分泌细胞中秘密颗粒的大小。基于这些发现，我们进一步研究了大鼠产后发育过程中格拉氏蛋白表达水平和垂体性促性腺促性腺瘤的超微结构的变化。直到出生后3周，在Pitutai促性腺激素的超微结构中尚未发现与性别相关的显着差异。两性在出生时两性的促性腺激素只有几个小型的秘密颗粒，以及逐渐大尺寸的秘密颗粒，这些颗粒含有CGA。然而，自出生后3周以来，雄性和雌性大鼠之间的Pitutai促性腺激素的差异变得显着。在此时间点之后，含有CGA的大型颗粒从雌性促性腺激素中消失了，而这些颗粒保留在雄性促性腺激素中。这些发现表明，在性成熟开始后，CGA的表达受到性立体的调节，从而导致与性别相关的分泌颗粒的组成和超微结构的差异。这些发现表明，花岗岩的表达水平影响了分泌花岗岩的超结构和大小的分泌花岗岩的大小。需要进一步研究每种花岗蛋白表达的调节机制，以阐明秘密内分泌细胞中秘书颗粒的多样性。较少的

项目成果

Immunocytochemical localization of secretogranin III in the endocrine pancreas of male rats.

• DOI: 10.1679/aohc.67.57
• 发表时间: 2004-03
• 期刊: Archives of histology and cytology
• 作者: Y. Sakai;M. Hosaka;A. Yoshinaga;Y. Hira;T. Harumi;Tsuyoshi Watanabe

Hosaka M. et al.: "Identification of a chromogranin A domain that mediates binding to secretogranin III and targeting to secretory granules in pituitary cells and pancreatic β-cells"Mol.Biol.Cell. 13. 3388-3399 (2002)

Hosaka M.等人：“介导与分泌颗粒蛋白III结合并靶向垂体细胞和胰腺β细胞中的分泌颗粒的嗜铬粒蛋白A结构域的鉴定”Mol.Biol.Cell.13.3388-3399(2002)。

Sakai Y. et al.: "Immunocytochemical localization of secretogranin III in the anterior lobe of male rat pituitary glands"J.Histochem.Cytochem.. 51. 227-238 (2003)

Sakai Y.等人：“雄性大鼠垂体前叶中分泌粒素 III 的免疫细胞化学定位”J.Histochem.Cytochem.. 51. 227-238 (2003)

Hosaka M. et al.: "Secretogranin III binds to cholesterol in the secretory granule membrane as an adapter for chromogranin A"J.Biol.Chem.. 279. 3627-3634 (2004)

Hosaka M. 等人：“Secretogranin III 作为嗜铬粒蛋白 A 的接头与分泌颗粒膜中的胆固醇结合”J.Biol.Chem.. 279. 3627-3634 (2004)

Immunocytochemical localization of secretogranin III in the anterior lobe of male rat pituitary glands.

雄性大鼠垂体前叶分泌颗粒素 III 的免疫细胞化学定位。

• 发表时间: 2003
• 期刊: J.Histochem.Cytochem. 51
• 作者: Sakai Y.;et al.
• 通讯作者: et al.