Function of stromal cell and follicular dendritic cells on the functional differentiation and formation of lymph node

基质细胞和滤泡树突状细胞对淋巴结功能分化和形成的作用

• 批准号: 14570007
• 负责人: YOSHIDA Toshiko
• 金额: $ 2.24万
• 依托单位: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570007/
• 关键词: IL-2 receptor; γ chain; knockout; mouse; stromal cell; Follicular dendritic cell(FDC); immnohistochemistry; lymphoid organ; IL-2 receptor γ chain; Knocout mouse; FDC-M1; FDC-M2; co-culture; リンパ節; 胚中心; IL-2 receptor g chain; Knockout

The relationship with stromal cells and follicular dendritic cells, which are considered to influence the structure of lymph node and the adjustment of microenvironment, was investigated used by IL-2 receptorγ chain knockout mouse.We had observed the ultrastructure by the electron microscopy. And the peculiar characteristic for the cell was detected by using monoclonal antibody, that is MOMA-1,MOMA-2,TCR-β chain,CD45R,MIDC-8,FDC-M1 and FDC-M2, for immuno histochemistry.1.The IL-2 Rγ chain did not lead the decrease the numbers of TcRβ(+) T cells, but decrease CD45R(+) B cells in the lymph node and spleen. It dose not influence the MIDC-8 positive dendritic cells which were located in the T cell area even if the structure of lymph node is not complete. The numbers of monocyte were increased, but MOMA-1(+) cells in spleen were disappeared, but much ones in lymph node. Precursor of FDCs was included in a group of stromal cells in this mouse. Because after the stimulation by the antigen, there was observed typical FDC elongated cytoplasmic processes with antigens between lymphocytes. The B cell did not play critical role to differentiate from immature FDC to mature.2.The stromal cells separated from IL-2R KO mouse were cultured with memory T cell, memory B cell and immune complexes. When we used the method of the separation FDC from immature lymph node which is difficult to separate from T cell areas and B cell area, we could get the precursor cellsOur result suggested that IL-2 receptorγ chain may do not play critical roles on the differentiation of FDC. This model is very useful for investigation of functional construction and microenvironment of stromal cells and FDC.

采用IL-2受体γ链敲除小鼠研究基质细胞和滤泡树突状细胞与淋巴结结构和微环境调节的关系，并通过电镜观察其超微结构。并用单克隆抗体进行免疫组织化学检测，发现细胞特有的特征，即MOMA-1、MOMA-2、TCR-β链、CD45R、MIDC-8、FDC-M1和FDC-M2。 1、IL-2 Rγ链不导致TcRβ(+) T细胞数量减少，但减少淋巴结中CD45R(+) B细胞数量。 和脾。即使淋巴结结构不完整，也不影响位于T细胞区的MIDC-8阳性树突状细胞。脾脏中单核细胞数量增多，但MOMA-1(+)细胞消失，而淋巴结中MOMA-1(+)细胞增多。 FDC 的前体包含在该小鼠的一组基质细胞中。因为在抗原刺激后，观察到典型的FDC拉长的细胞质突起，淋巴细胞之间有抗原。 B细胞在未成熟FDC向成熟分化过程中不发挥关键作用。2.将IL-2R KO小鼠分离的基质细胞与记忆T细胞、记忆B细胞和免疫复合物一起培养。当我们采用从T细胞区和B细胞区难以分离的未成熟淋巴结中分离FDC的方法时，我们可以获得前体细胞。我们的结果表明IL-2受体γ链可能在FDC的分化中不起关键作用。该模型对于研究基质细胞和FDC的功能结构和微环境非常有用。