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Analysis of ontogeny of circadian rhythm and the transmission of circadian-rhythm-information

昼夜节律的个体发育和昼夜节律信息的传递分析

基本信息

批准号：
14570063
负责人：
ISOBE Yoshiaki
金额：
$ 2.24万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570063/
关键词：
Circadian rhythm Clock controlled gene Per Bmal1 vasopressin Melatonin suprachiasmatic nucleus Pineal gland Bmal1 vasopressin

项目摘要

We investigated 1)ontogeny of the circadian rhythm in the suprachiasmatic nucleus (SCN), a center of biological rhythm, and 2)transmission of circadian-rhythm-information from the SCN to the other brain area. A clock mechanism in the SCN known to involve a transcriptional negative feedback loop in which CLOCK and BMAL1 function as positive regulators and three period gene products play a role in negative feedback. 1)To clarify the maternal effects on the ontogeny of circadian rhythm, we measured the circadian rhythm of rPer2, rBmla1 and rAVP (arg-vasopressin, an output signal transmitter from the SCN) mRNAs in the SCN of sighted rat-pups nursed under the light-dark (LD) cycle and nursed under the reversed LD (DL) cycle (two groups) which were i) nursed from the day of birth and ii) 6 days after birth) or under the constant dim light (DD), and kept. The m RNAs were determined using RT-PCR and normalized by beta-actin mRNA in each sample. The rPer2, rBmal1 and rAVP mRNA rhythmicity were … More free run during the nursing period even under the LD entrained condition. At the 2nd week of age, environmental lighting cycle with the dam co-operate to entrain the pup's rhythm. The rBmal1 transcriptional activation by PER2 might be functioning after the maturation of clock mechanisms. The results indicate that the nursing dam partially entrains pup's circadian rhythm. 2)To know the transduction system of circadian signals from the SCN to the pineal gland, we determined the effects of melatonin injection on (1 mg/kg, i.p.) rPer2 and AVP mRNAs in the punched out SCN, PVN and pineal gland of rats by using PT PER as in 1). AVP peptide contents were also measured in SCN and PVN. PVN. In the SCN, rPer2 content increased at both light and dark period, however no fluctuations were shown in the AVP mRNA. In the PVN, rPer2 decreased and increased at light and dark period, respectively. In the pineal gland, rPer2 mRNA increased at 60 after the melatonin injection. Recently, the melatonin-AVP reciprocal systems. The results might indicate that PVN is temporally coupled with the SCN clock activity.We clarified the development of circadian rhythm in the suprachiasmatic nucleus and the output mechanisms from the SCN, at least in part. Less

我们研究了1）在视交叉上核（SCN），生物节律的中心，昼夜节律的个体发生，和2）从SCN到其他脑区的昼夜节律信息的传输。SCN中的时钟机制已知涉及转录负反馈环，其中CLOCK和BMAL 1作为正调节因子发挥作用，三个周期的基因产物在负反馈中发挥作用。1）为了阐明母体对昼夜节律个体发育的影响，我们测定了rPer 2、rBmla 1和rAVP的昼夜节律（Arg-加压素，来自SCN的输出信号传递器）在光暗（LD）周期下喂养和在反向LD（DL）周期下喂养的有视力的大鼠幼仔的SCN中的mRNA（两组）分别从出生当天开始和出生后6天开始喂养，或在恒定的昏暗灯光下（DD），并保持。使用RT-PCR测定mRNA，并通过每个样品中的β-肌动蛋白mRNA进行标准化。rPer 2、rBmal 1和rAVP mRNA的节律性分别为： ...更多信息即使在LD夹带的情况下，在护理期间也可以自由奔跑。在第2周龄时，环境照明周期与母鼠合作，引导幼鼠的节奏。PER 2对rBmal 1转录的激活作用可能是在生物钟机制成熟后发挥作用的。结果表明，哺乳母鼠部分地影响了幼鼠的昼夜节律。2）为了解SCN向松果体传递昼夜节律信号的途径，本实验观察了褪黑素（1 mg/kg，i. p.）使用如1）中的PT PER，在大鼠的SCN、PVN和松果体中冲压出rPer 2和AVP mRNA。同时测定SCN和PVN中AVP肽含量。PVN。在SCN中，rPer 2含量在光照和黑暗期间均增加，而AVP mRNA无明显变化。在PVN中，rPer 2在光照和黑暗期分别降低和升高。在松果体中，注射褪黑素后60分钟rPer 2 mRNA增加。最近，褪黑激素-AVP相互作用系统。这些结果表明，视交叉上核的昼夜节律与视交叉上核的时钟活动存在时间上的耦合，我们至少部分地阐明了视交叉上核昼夜节律的形成和视交叉上核的输出机制。少