Relationship of circadian rhythm with cycle control

在脊椎动物器官中存在着两个自振荡系统：昼夜节律和细胞周期。利用E14大鼠胎脑视神经上皮分散细胞培养制备增殖神经球。第二次传代后，于9:00、15:00、21:00、03:00每隔6小时采集细胞。9:00时g1细胞比例小于21:00时。Per2和p27 mrna表现出相似的昼夜节律，在21:00达到峰值。从结果来看，细胞周期受到昼夜节律成分的严格控制。

Two self-oscillating systems are running in vertebrate organ that are circadian rhythm and cell cycle. Proliferating neurosphere was prepared from the dispersed cell culture of preoptic neuroepitherium in E14 rat fetus brain. After the second passage, the cells were collected 6 hr intervals at 9:00, 15:00, 21:00 and 03:00. Proportion of G1cells at 9:00 was smaller than that at 21:00. Per2 and p27 mRNAs showed similar circadian rhythms, which peaked at 21:00. From the results, cell cycle is tightly controlled by the component of circadian rhythm.