IN SEARCH OF THE COUSE AND TREAMENT FOR JETLAG

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1.Dichotomy of the circadian center is associated with jet lag syndromeDissociation of the suprachiasmatic nucleus, the circadian center, into two oscillators was observed after 10 hour delay and 6 hour advance of light-dark cycle. The ventrolateral region of the SCN(VLSCN) shifted rapidly whereas the dorsomedial regions of the SCN(DMSCN) shifted slowly, spent more than a week to regain synchronization of the VLSCN and DMSCN. The sluggish shift of the DMSCN corresponded to the suppressed locomotors activity during the subjective night, which suggested that jet lag was caused by the slow shift of the DMSCN after LD cycle shift.2.A Transcription Factor Response Element for Gene Expression During Circadian NightWe profiled suprachiasmatic nuclei(SCN) and liver genome-wide expression patterns under light/dark(LD) cycles and constant darkness(DD). We determined transcription start sites(TSS) of human orthologues for newly identified cycling genes and then performed bioinformatical searches for relationships between time-of-day specific expression and transcription factor response elements around TSS. We demonstrate the role of the Rev-ErbA/ROR response element in gene expression of nocturnally expressed genes in SCN and liver.3.Phosphodiesterase type 4(PDE4) specifically degrade cAMPTo elongate Per1 expression by the inhibition of cAMP degradation, we used Rolipram, a specific inhibitor of phosphodiesterase type 4, which degrades the cAMP. With Rolipram application, the amount of Perl transcripts increased transiently but the period length of Perl induction was not extended.