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The role of eisonphil chemotactic cytokine in parasitic infections

eisonphil趋化细胞因子在寄生虫感染中的作用

基本信息

批准号：
14570214
负责人：
OHASHI Makoto
金额：
$ 2.18万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

ECF-L is the eosinophil chemotactic cytokine that belong to the chitinase family proteins. In the present study, we identified the role of eosinophil chemotactic cytokine ECF-L in Schistosoma or Mesocestoides-infected mouse. Extra-medulla hemopoietic focii of eosinophilic linage were observed around eosinophilic granulomatous lesions in the liver upon infection with those tissue-dwelling parasites. Immature eosinophils with donut-type ring nuclei were generally observed in those extra-medulla hemopoietic foci, whereas mature eosinophil with polymorphic nuclei were generally observed in the eosinophilic granulomatous lesions, indicating that those eosinophils were recruited there by chemotactic factors.In order to clarify the role of ECF-L in parasitic infections, we employed the time-course study of the granulomatous lesion formation in schistosomiasis japonicum and schistosomiasis mekongi. When eosinophil chemotactic activity of the culture supernatants of the granulomatous lesions fr … More om S. japonicum or S. mekongi-infected mice, the peak of the eosinophil chemotactic activity was observed at 6 week and at 15 wk post-infection, in S. japonicum infection and in S. mekongi infection, respectively. In S. japonicum infection, intense accumulation of inflammatory eosinophils were observed around the deposited eggs in the liver at 6 wk post-infection. In contrast, slower granuloma formation with many vacuoles were observed at 9-12 weeks after S. mekongi infection. At 15 weeks after infection, most of the vacuoles were disappeared from the granulomatous lesions. Thus, the production of ECF-L was closely related with the change of the feature of granulomatous lesions both in schistosomiasis japonicum and schistosomiasis mekongi. Furthermore, pre-incubation of eosinophils with chitinase A resulted in the increase of ECF activity to ECF-L or synthetic ECF-L peptides. These facts indicates that ECF-L acts not only chemoattractants but also the stimulator of eosinophils for the enhancement of leukotactic functions. Less

ECF-L是嗜酸性粒细胞趋化因子，属于几丁质酶家族蛋白。在本研究中，我们确定了嗜酸性粒细胞趋化细胞因子ECF-L在血吸虫和中囊蚴感染小鼠中的作用。这些组织寄生虫感染后，在肝脏嗜酸性肉芽肿病变周围观察到嗜酸性细胞系的髓外造血因子。在髓外造血灶中通常观察到具有环形核的未成熟嗜酸性粒细胞，而在嗜酸性肉芽肿性病变中通常观察到具有多态性核的成熟嗜酸性粒细胞，表明这些嗜酸性粒细胞在那里被趋化因子招募。我们对日本血吸虫和湄公血吸虫肉芽肿病变形成进行了时间进程研究。当肉芽肿性病变的培养上清液中嗜酸性粒细胞趋化活性降低时， ...更多信息奥姆山japonicum或S. mekongi感染的小鼠，嗜酸性粒细胞趋化活性的高峰出现在感染后6周和15周，S.日本血吸虫感染和S. Mekongi感染。In S.日本血吸虫感染后6周，在肝脏中沉积的虫卵周围观察到炎性嗜酸性粒细胞的强烈积聚。相反，S. mekongi感染感染后15周，大部分空泡从肉芽肿性病变中消失。因此，ECF-L的产生与日本血吸虫和湄公血吸虫肉芽肿病变特征的改变密切相关。此外，用几丁质酶A预孵育嗜酸性粒细胞导致ECF对ECF-L或合成ECF-L肽的活性增加。这些事实表明，ECF-L的行为不仅是化学引诱剂，但也刺激嗜酸性粒细胞的白细胞趋化功能的增强。少