The expression of hepsin in liver

Hepsin在肝脏中的表达

• 批准号: 14570516
• 负责人: FURUKAWA Midori
• 金额: $ 2.18万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570516/
• 关键词: hepsin; serine protease; CCl4; liver regeneration; LEC rat; 急性肝障害; 前立腺; CCl_4; ノックアウトマウス; 肝炎; 肝臓癌

Hepsin was originally found as a cDNA clone encoding a putative serine protease with a trypsin-like activity in a type II membrane topology. Its presence/access at the cell surface and in the intracellular microsome fraction are apparently required for growth and maintenance of morphology of cells in culture. But interestingly, hepsin-null mice showed no severely defective phenotype, indicating that it may not play any critical role in the development and at least in the early postnatal stage. To investigate the function of hepsin in liver in detail, CCl4 injection and 70% hepatectomy were proceeded. Hepsin mRNA level were decreased in both experiments on the early stage of acute inflammation and regeneration. In LEC rat, the same result was obtained in acute hepatitis stage, and also, hepsin expression in pre-cancer tissue is higher than that in cancer tissue.

Hepsin最初被发现为编码推定的丝氨酸蛋白酶的cDNA克隆，其在II型膜拓扑结构中具有胰蛋白酶样活性。其在细胞表面和细胞内微粒体部分的存在/进入显然是培养物中细胞生长和形态维持所需的。但有趣的是，hepsin-null小鼠没有表现出严重缺陷的表型，这表明它可能在发育中没有发挥任何关键作用，至少在出生后早期。为探讨肝蛋白酶在肝脏中的作用，采用四氯化碳（CCl_4）注射和70%肝切除术。在急性炎症和再生的早期阶段，Hepsin mRNA水平在两个实验中均降低。在LEC大鼠中，在急性肝炎阶段也得到相同的结果，并且癌前组织中hepsin的表达高于癌组织。