The role of hepsin, a cell-surfaceserine protease

Hepsin（一种细胞表面丝氨酸蛋白酶）的作用

• 批准号: 11670542
• 负责人: FURUKAWA Midori
• 金额: $ 2.3万
• 依托单位: Tokyo Woman's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670542/
• 关键词: hepsin; knock out; liver regeneration; hepatitis; HGF; LECラット

1 The role of hepsin on the regeneration of the liverTo study its function in vivo, we have disrupted the mouse hepsin gene by homologous recombination. Groups of hepsin -/- mice and wild-type mice were subjected to 70 % partial hepatectomy to induce liver regenerstion. Liver regeneration rate was changed in hepsin deficient mice.2 Hepsin and hepatitis, hepatocellular carcinomaThe Long-Evans Cinnamon (LEC) rat, when survives acute hepatitis, develops chronic hepatitis, and subsequently hepatocellular carcinoma. Hepsin mRNA were studied using the liver of LEC rat over the period of 1 year. Differences were clear in the rats with hepatitis and hepatocellular carcinoma.3 Relation of hepsin and HGF (hepatocyte growth factor)The levels of hepsin mRNA with or without HGF in the medium were studied by use of hepatoma cell line, HepG2 cells. No significant difference of hepsin expression was seen in this experiment.

1. hepsin在肝再生中的作用为了研究hepsin在体内的功能，我们利用同源重组的方法破坏了小鼠hepsin基因。对hepsin -/-小鼠和野生型小鼠组进行70%部分肝切除术以诱导肝再生。Hepsin缺陷小鼠的肝再生率发生变化。2 Hepsin与肝炎、肝细胞癌Long-Evans Cinnamon（LEC）大鼠在急性肝炎后存活，发展为慢性肝炎，随后发展为肝细胞癌。用LEC大鼠肝脏研究Hepsin mRNA在1年期间的变化。在肝炎和肝癌大鼠中差异明显。3 hepsin和HGF（肝细胞生长因子）的关系通过使用肝癌细胞系HepG 2细胞研究了培养基中有或没有HGF的hepsin mRNA的水平。在本实验中没有观察到hepsin表达的显著差异。