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The amino acid transporter and gene expresstion during healing of gastric ulcer

胃溃疡愈合过程中氨基酸转运蛋白及基因表达

基本信息

批准号：
14570517
负责人：
KATO Kimitoshi
金额：
$ 1.73万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570517/
关键词：
amino acid transporter LAT1 COX carcinoid colon cancer esophageal cancer gene expression gastric ulcer ストレス潰瘍遺伝子発現解析潰瘍治癒

项目摘要

We evaluate the amino acid transport transporters and the expressions of cyclooxygenase(COX)-1 and -2, and their interactions in the healing process of peptic ulcer or mucosal injury. COX-2 immunoreactivities were increased in interstitial tissue, granulation tissue and regenerative epithelial cells at the ulcer margin in rat acetic acid-induced ulcer. After ulcer induction, rats were treated for one week with indomethacin, a COX inhibitor, ulcer healing was significantly delayed and the immunohistochemical expression of LAT1, a Na^+-independent neutral amino acid transporter was significantly higher than that in controls.These findings suggest LAT1 were PG independently expressed and to be involved in for cell growth, the ulcer repair process. We immunohistochemically studied COX-1 and -2 expressions in gastrointestinal carcinoids and their metastases (lymph node, liver). COX-2 was expressed in all gastrointestinal carcinoids as well as their metastases. The staining pattern was diffu … More se and cytoplasmic. Strong immunoreactivity was also noted in the area of the tumor displaying stronnal invasion into the submucosa. On the other hand, faint COX-1 expression was detected in only some carcinoids. The moderate to strong immunoreactivity of LAT1 was observed in carcinoids. COX-2 and LAT1 may play a more important role in tumor growth of carcinoids. The expression of LAT1 by immunostaing was observed in the rat liver metstatic nodules of colon cacner cell and the intensity of LAT1 expression was correlative to the tumor size. We conclude that LAT1 plays an important role of liver metastatic tumors and blocking agents for LAT1 will be useful for suppressing the tumor proliferation. Esophageal squamous cell carcinoma expressed LAT1 throughout the tumor. LAT1 expression in esophageal squamous cell carcinoma was significantly higher than that in non-cancerous esophageal mucosa. LAT1 expression in esophageal carcinoma increased as the depth of invasion progressed, and as the tumor size increased. The results suggest that LAT1 plays a significant role in cell proliferation, differentiation and invasion in esophageal squamous cell carcinoma.Altered gene expressions of heat shock proteins, cell cycle regulators, protooncogenes and metabolic enzymes were recognized in the stomachs of water immersion-restraint stress (WIRS) rats using a high-density oligonucleotide array. p38 mitogen-activated protein kinase, did not reportedly show gastric expression changes in response to stress. Less

我们探讨了氨基酸转运载体和环氧合酶(COX)-1、-2在消化性溃疡或粘膜损伤愈合过程中的表达及其相互作用。在大鼠醋酸诱导的溃疡模型中，溃疡边缘的间质组织、肉芽组织和再生上皮细胞中COX-2免疫反应增强。溃疡诱导后，给予COX抑制剂消炎痛1周后，溃疡愈合明显延迟，中性氨基酸转运体LAT1的免疫组织化学表达显著高于对照组，提示LAT1为PG非依赖性表达，参与了细胞生长、溃疡修复过程。我们用免疫组织化学方法研究了COX-1和COX-2在胃肠道类癌及其转移灶(淋巴结、肝脏)中的表达。COX-2在所有胃肠道类癌及其转移灶中均有表达。染色图案为弥漫…更多的Se和细胞质。肿瘤侵犯粘膜下层的区域也有较强的免疫反应。而COX-1仅在部分类癌组织中有微弱表达。LAT1在类癌中呈中到强免疫反应。COX-2和LAT1可能在类癌的肿瘤生长中发挥更重要的作用。免疫组织化学方法观察到LAT1在大鼠结肠癌肝转移结节中的表达，其表达强度与肿瘤大小有关。结论：LAT1在肝转移瘤中起重要作用，LAT1的阻断剂将有助于抑制肿瘤的增殖。食管鳞癌LAT1在整个肿瘤中均有表达。LAT1在食管鳞癌组织中的表达明显高于非癌组织。LAT1在食管癌中的表达随着肿瘤侵袭深度的增加和肿瘤大小的增大而增加。结果表明，LAT1在食管鳞癌的细胞增殖、分化和侵袭中起重要作用。应用高密度寡核苷酸芯片技术，在水浸束缚应激(WIRS)大鼠的胃中发现了热休克蛋白、细胞周期调节因子、原癌基因和代谢酶基因表达的改变。P38丝裂原活化蛋白激酶，据报道没有显示出应激反应中胃部表达的变化。较少