The amino acid transporter and gene expresstion during healing of gastric ulcer

我们评估了氨基酸转运转运蛋白和环氧化酶(COX)-1和-2的表达，以及它们在消化性溃疡或粘膜损伤愈合过程中的相互作用。乙酸诱导大鼠溃疡间质组织、肉芽组织和溃疡边缘再生上皮细胞中COX-2免疫反应性升高。溃疡诱导后，大鼠给予COX抑制剂吲哚美辛治疗1周，溃疡愈合明显延迟，Na^+非依赖性中性氨基酸转运体LAT1的免疫组化表达明显高于对照组。这些发现表明LAT1是PG独立表达的，并参与细胞生长，溃疡修复过程。我们用免疫组织化学方法研究了COX-1和cox -2在胃肠道类癌及其转移灶（淋巴结、肝脏）中的表达。COX-2在所有胃肠道类癌及其转移灶中均有表达。染色呈弥漫性，胞浆性染色。强烈的免疫反应性也被发现在肿瘤区域显示强烈侵袭粘膜下层。另一方面，仅在部分类癌中检测到微弱的COX-1表达。在类癌中观察到LAT1具有中强的免疫反应性。COX-2和LAT1可能在类癌的肿瘤生长中发挥更重要的作用。免疫染色法观察了大鼠结肠癌细胞转移性肝结节中LAT1的表达，其表达强度与肿瘤大小相关。我们得出结论，LAT1在肝转移瘤中发挥重要作用，LAT1的阻断剂将有助于抑制肿瘤的增殖。食管鳞状细胞癌在整个肿瘤中表达LAT1。食管鳞状细胞癌中LAT1的表达明显高于非癌性食管黏膜。在食管癌中，LAT1的表达随浸润深度和肿瘤大小的增加而增加。结果提示，LAT1在食管鳞状细胞癌的细胞增殖、分化和侵袭中起重要作用。利用高密度寡核苷酸阵列研究了水浸抑制应激（WIRS）大鼠胃中热休克蛋白、细胞周期调节因子、原癌基因和代谢酶基因表达的改变。P38丝裂原活化蛋白激酶在应激反应中未见胃表达变化。少

We evaluate the amino acid transport transporters and the expressions of cyclooxygenase(COX)-1 and -2, and their interactions in the healing process of peptic ulcer or mucosal injury. COX-2 immunoreactivities were increased in interstitial tissue, granulation tissue and regenerative epithelial cells at the ulcer margin in rat acetic acid-induced ulcer. After ulcer induction, rats were treated for one week with indomethacin, a COX inhibitor, ulcer healing was significantly delayed and the immunohistochemical expression of LAT1, a Na^+-independent neutral amino acid transporter was significantly higher than that in controls.These findings suggest LAT1 were PG independently expressed and to be involved in for cell growth, the ulcer repair process. We immunohistochemically studied COX-1 and -2 expressions in gastrointestinal carcinoids and their metastases (lymph node, liver). COX-2 was expressed in all gastrointestinal carcinoids as well as their metastases. The staining pattern was diffu … More se and cytoplasmic. Strong immunoreactivity was also noted in the area of the tumor displaying stronnal invasion into the submucosa. On the other hand, faint COX-1 expression was detected in only some carcinoids. The moderate to strong immunoreactivity of LAT1 was observed in carcinoids. COX-2 and LAT1 may play a more important role in tumor growth of carcinoids. The expression of LAT1 by immunostaing was observed in the rat liver metstatic nodules of colon cacner cell and the intensity of LAT1 expression was correlative to the tumor size. We conclude that LAT1 plays an important role of liver metastatic tumors and blocking agents for LAT1 will be useful for suppressing the tumor proliferation. Esophageal squamous cell carcinoma expressed LAT1 throughout the tumor. LAT1 expression in esophageal squamous cell carcinoma was significantly higher than that in non-cancerous esophageal mucosa. LAT1 expression in esophageal carcinoma increased as the depth of invasion progressed, and as the tumor size increased. The results suggest that LAT1 plays a significant role in cell proliferation, differentiation and invasion in esophageal squamous cell carcinoma.Altered gene expressions of heat shock proteins, cell cycle regulators, protooncogenes and metabolic enzymes were recognized in the stomachs of water immersion-restraint stress (WIRS) rats using a high-density oligonucleotide array. p38 mitogen-activated protein kinase, did not reportedly show gastric expression changes in response to stress. Less