The effects of estrogen like agents on cardio-protection via induction of stress proteins

雌激素样药物通过诱导应激蛋白对心脏保护的作用

• 批准号: 14570702
• 负责人: NISHIDA Masashi
• 金额: $ 1.92万
• 依托单位: Kobe College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570702/
• 关键词: Estrogen; Cardiac myocyte; Hydrogen peroxide; Cell injury; 分化; 修復; 再生

Sex difference is one of the major factors that modulate pathogenesis of heart diseases. Especially in ischemic heart diseases, anti-atherogenic actions of estrogen were examined extensively. However, the action of estrogen on cardiac myocytes per sue was not elucidated yet. Therefore, we examined the effect of 17β-estradiol on cultured cardiac myocytes. We utilized P19CL6 cell line as cardiac progenitor system and differentiated them into cardiac phenotype with DMSO. 17β-estradiol induced manganese superoxide dismutase in differentiated P19CL6 cells and increased tolerance of the cells to hydrogen peroxide. We also examined whether 176-estradiol promote differentiation of P19CL6 cells into cardiac phenotype. P19CL6 cells treated with DMSO started spontaneous beating earlier in the presence of 176-estradiol compared to control cells without estradiol. These results indicate that 17β-estradiol causes cardioprotective action directly on cardiac myocytes through the increase in the tolerance of myocytes to oxidative stress and the promotion of myocyte regeneration from progenitor cells.

性别差异是影响心脏病发病的主要因素之一。特别是在缺血性心脏病中，雌激素的抗动脉粥样硬化作用被广泛研究。但雌激素对心肌细胞的作用尚不清楚。因此，我们研究了17β-雌二醇对培养的心肌细胞的作用。我们利用P19 CL 6细胞系作为心脏祖细胞系，用DMSO诱导其分化为心脏表型。17β-雌二醇诱导分化的P19 CL 6细胞产生锰超氧化物歧化酶，并增强细胞对过氧化氢的耐受性。我们还研究了176-雌二醇是否促进P19 CL 6细胞分化为心脏表型。与没有雌二醇的对照细胞相比，在176-雌二醇存在下，用DMSO处理的P19 CL 6细胞更早地开始自发搏动。这些结果表明，17β-雌二醇通过增加心肌细胞对氧化应激的耐受性和促进心肌细胞从祖细胞再生，直接对心肌细胞产生心脏保护作用。

项目成果

西田昌司, 別井美穂: "成熟マウス心筋細胞の単離と模擬虚血モデルの作成"神戸女学院大学論集. 50. 199-210 (2003)

Masashi Nishida、Miho Betsui：“成熟小鼠心肌细胞的分离和模拟缺血模型的创建”神户女学院大学杂志 50. 199-210 (2003)。

西田昌司, 別井美穂: "成熟マウス心筋細胞の単離と模擬虚血モデルの作成"神戸女学院大学論集. 50. 197-210 (2003)

Masashi Nishida、Miho Betsui：“成熟小鼠心肌细胞的分离和模拟缺血模型的创建”神户女学院大学杂志 50. 197-210 (2003)。

西田昌司, 近藤奈津子: "女性ホルモンエストロゲンとカテコラミンの血管形成に与える影響〜培養血管内皮細胞を用いた検討〜"神戸女学院大学論集. (印刷中). (2004)

Shoji Nishida、Natsuko Kondo：“女性荷尔蒙雌激素和儿茶酚胺对血管生成的影响 - 使用培养的血管内皮细胞的研究 -”神户女学院大学杂志（2004 年出版）。

Nishida Masashi, Natsuko Kondo: "The effects of estrogen and catecholamine in angiogenesis models of vascular endothelial cells."Kobe College Studies. (in press). (2004)

Nishida Masashi、Natsuko Kondo：“雌激素和儿茶酚胺对血管内皮细胞血管生成模型的影响。”神户学院研究。

Zhang H, Toyofuku T, Kamei J, Hori M: "GATA-4 regulates cardiac morphogenesis through transactivation of the N-cadherin gene."Biochem Biophys Res Commun.. 312. 1033-1038 (2003)

张 H、Toyofuku T、Kamei J、Hori M：“GATA-4 通过 N-钙粘蛋白基因的反式激活调节心脏形态发生。”Biochem Biophys Res Commun.. 312. 1033-1038 (2003)