Renoprotective role of interstitial macrophages in the evolution of renal fibrosis

间质巨噬细胞在肾纤维化演变中的肾脏保护作用

• 批准号: 15591124
• 负责人: NISHIDA Masashi
• 金额: $ 2.24万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591124/
• 关键词: renal fibrosis; macrophage; unilateral ureteral obstruction; unilateral ureteral obstruction

We performed adoptive transfer of bone marrow-derived(BM) macrophages following pharmacological depletion of leukocytes in a mouse model of unilateral ureteral obstruction(UUO), a well-established experimental model of progressive renal interstitial fibrosis. Treatment with cyclophosphamide(CPM) caused marked decrease in the numbers of F4/80-positive interstitial macrophages as well as in peripheral blood leukocyte counts, and adoptive transfer of BM macrophages to CPM-treated mice resulted in significant increase in the numbers of interstitial macrophages both at day 5 and at day 14 after UUO. At day 5 after UUO, no significant change was observed in the degree of renal interstitial fibrosis either by treatment with CPM or with CPM+macrophage. However, at day 14 after UUO, treatment with CPM caused significant increase in the degree of interstitial fibrosis, and adoptive macrophage transfer to these mice attenuated this enhancement in renal fibrosis. Our result suggests the role of infiltrating macrophages on facilitating tissue repair at late stage of UUO.

我们在单侧输尿管梗阻(UUO)的小鼠模型上进行了骨髓来源的巨噬细胞的过继移植，这是一个公认的进行性肾间质纤维化的实验模型。环磷酰胺(CPM)治疗后F4/80阳性的间质巨噬细胞数和外周血白细胞数显著减少，过继转移BM巨噬细胞到CPM治疗组小鼠的间质巨噬细胞数在UUO后第5天和第14天显著增加。UUO后第5天，CPM或CPM+巨噬细胞治疗对肾间质纤维化程度无明显影响。然而，在UUO后第14天，CPM治疗导致间质纤维化程度显著增加，过继巨噬细胞转移到这些小鼠身上减弱了这种肾脏纤维化的增强。我们的结果提示巨噬细胞在UUO晚期促进组织修复中的作用。

项目成果

Renoprotective effect of macrophage angiotensin II type 1 receptor on the evolution of renal fibrosis

巨噬细胞血管紧张素II 1型受体对肾纤维化演变的肾脏保护作用

• 发表时间: 2004
• 期刊: Pediatr Nephrol 19(11)
• 作者: Nishida M;Hamaoka K;Fujinaka H et al.
• 通讯作者: Fujinaka H et al.

Effect of hematopoietic cytokines on renal function in cisplatin-induced ARF in mice

• DOI: 10.1016/j.bbrc.2004.09.051
• 发表时间: 2004-11-05
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Nishida, M;Fujimoto, SI;Hamaoka, K
• 通讯作者: Hamaoka, K

Renal tubular regeneration by bone marrow-derived cells in a girl after bone marrow transplantation.

女孩骨髓移植后骨髓来源细胞的肾小管再生。

• 发表时间: 2003
• 期刊: Am J Kidney Dis 42(5)
• 作者: Nishida M;Kawakatsu H;Shiraishi I et al.
• 通讯作者: Shiraishi I et al.

Nishida M, Kawakatsu H, Shiraishi I et al.: "Renal tubular regeneration by bone marrow-derived cells in a girl after bone marrow transplantation"Am J Kidney Dis. 42(5). E10-E12 (2003)

Nishida M、Kawakatsu H、Shiraishi I 等：“骨髓移植后女孩骨髓来源细胞的肾小管再生”Am J Kidney Dis。