Analysis of the roles of dendritic cell in chronic gastritis and gastric MALT lymphoma

树突状细胞在慢性胃炎和胃MALT淋巴瘤中的作用分析

• 批准号: 14571004
• 负责人: MORI Shinichiro
• 金额: $ 1.86万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571004/
• 关键词: Dendritic cell; Gastric MALT lymphoma; Th polarization; Chronic gastritis; MALTリンパ腫; 低悪性度MALTリンパ腫; T細胞

We studied 8 cases of chronic gastritis and 6 cases of gastric MALT lymphoma to evaluate the distribution of dendritic cell(DC) by immunohistochemical analysis with DC specific monoclonal antibody ; fascin. We observed fascin-positive DC in chronic gastritis and gastric MALT lymphoma. All cases of gastric MALT lymphoma showed presence of fascin-positive DC. However, 5 cases of chronic gastritis showed no involvement of fascin-positive DC. DC were mainly presented within T cell infiltrating interfollicular zone in gastric MALT lymphoma. Fascin-positive DC were expressed CD1a-,CD4-,CD21-,DC-LAMP+,IL-3R-. These finding suggested DC in gastric MALT lymphoma were mainly myeloid lineage DC. Cytokines producing by DC is key role in Th balance. Therefore, we performed immunohistochemical analysis of cytokines expression in MALT lymphoma. In 3 cases of gastric MALT lymphoma we observed no expression of IL-12,IFN-α, and IL-4. We detected only IFN-y expression in all cases. These findings suggested that gastric MALT lymphoma might be Th1 disease.

对8例慢性胃炎和6例胃MALT淋巴瘤进行了树突状细胞(DC)的免疫组织化学染色。在慢性胃炎和胃MALT淋巴瘤中，我们观察到了Fasin阳性的DC。所有胃MALT淋巴瘤均可见Fasin阳性树突状细胞。而5例慢性胃炎患者均未见筋膜蛋白阳性DC受累。在胃MALT淋巴瘤中，树突状细胞主要分布在浸润性滤泡间带。Fasin阳性DC表达CD1a-、CD4-、CD21-、DC-LAMP+、IL-3R-。提示胃MALT淋巴瘤DC主要为髓系树突状细胞。DC产生的细胞因子在Th平衡中起着关键作用。因此，我们对细胞因子在MALT淋巴瘤中的表达进行了免疫组织化学分析。3例胃MALT淋巴瘤中未见IL-12、干扰素-α和IL-4的表达。我们在所有病例中仅检测到干扰素-γ的表达。提示胃MALT淋巴瘤可能为Th1病。