Cloning of human specific prorenin convertase

长期以来，人们一直不清楚原肾素真正转化为肾素的情况，肾素是该酶的活性形式。到目前为止，腺激肽释放酶(PRECE)、athepsin B、PC1/3和PC5/6被认为是候选酶。然而，对于真正的酶来说，特异性和活性是不够的。本项目旨在以肾RNA为底物，以简并引物聚合酶链式反应为基础，克隆和鉴定Kex家族方法中的真原蛋白转换酶。获得的克隆是已知的克隆。因此，到目前为止，我们认为肾脏中不存在Kex家族中的原肾素转换酶。

For long time, it has been unclear about genuine convertase for prorenin to renin, active form of the enzyme. Until now, glandular kallikrein (PRECE), athepsin B, PC1/3 and PC5/6 were regarded as the candidate enzymes. However, the specificity and activity were not enough for the genuine enzyme. This project aimed the cloning and characterization of the genuine prorenin convertase with renal RNA substraction and degenerate primer PCR for Kex family method. Gained clones were known ones. So that, as conclusion, so far, it is thought that there exist no prorenin convertase in Kex family in kidney.