Cloning of human specific prorenin convertase

人特异性肾素原转化酶的克隆

• 批准号: 14571020
• 负责人: KONOSHITA Tadashi
• 金额: $ 1.86万
• 依托单位: Faculty of Medical Sciences, University of Fukui
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571020/
• 关键词: renin; prorenin; convertase

For long time, it has been unclear about genuine convertase for prorenin to renin, active form of the enzyme. Until now, glandular kallikrein (PRECE), athepsin B, PC1/3 and PC5/6 were regarded as the candidate enzymes. However, the specificity and activity were not enough for the genuine enzyme. This project aimed the cloning and characterization of the genuine prorenin convertase with renal RNA substraction and degenerate primer PCR for Kex family method. Gained clones were known ones. So that, as conclusion, so far, it is thought that there exist no prorenin convertase in Kex family in kidney.

长期以来，人们一直不清楚肾素原转化为肾素（该酶的活性形式）的真正转化酶。迄今为止，腺激肽释放酶（PRECE）、athepsin B、PC1/3和PC5/6被认为是候选酶。然而，对于真正的酶来说，特异性和活性还不够。该项目旨在通过 Kex 家族方法的肾 RNA 消减和简并引物 PCR 来克隆和表征真正的肾素原转化酶。获得的克隆是已知的。因此，作为结论，目前认为肾脏中不存在Kex家族的肾素原转化酶。