Neointimal formation in the earotid artery of in IP3^R knock out mouse

IP3^R 敲除小鼠耳动脉中的新内膜形成

• 批准号: 14571129
• 负责人: NAKAZAWA Tatsu
• 金额: $ 2.05万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571129/
• 关键词: intival hyperplasia; endothelial cell; smooth muscle cell; signal pathway; 静脈置換モデル

Vascular remodeling with neointimal formation was studied in 1P3-R knock out mouse. IP3-R type1 hetero knock out mouse (n=10)、 IP-3 type2 homo knock out mouse (n=9)、 IP-3 type3 homo knock out mouse (n=11) and control mouse (n=10) were used in the carotid ligation model. The animals were anesthetized, and the left common carotid artery was exposed through a small midline incision in the neck. The artery was completely ligated just proximal to the carotid bifurcation to disrupt blood flow. Left carotids were snap-frozen at days 28 after ligation. Neointima area/medial area ratio was calculated. The maximum ratio of IP3-type1 and type2 homo knock out mouse group was significantly smaller than that of control. The average ratio of IP-3 type2 and type3 homo knock out mouse group was significantly smaller than that of control. In this study, intimal hyperplasia in the carotid ligation model was suppressed in IP3-R knockout mouse.

研究了1P3-R基因敲除小鼠血管重塑与新生内膜形成的关系。颈动脉结扎模型采用IP3-R型异源基因敲除小鼠(n=10)、、IP-3型基因敲除小鼠(n=9)、、IP-3型基因敲除小鼠(n=11)和对照组(n=10)。动物麻醉后，通过颈部正中小切口暴露左侧颈总动脉。动脉被完全结扎在颈动脉分叉处的近端，以扰乱血流。结扎后第28天快速冰冻左侧颈总动脉。计算新生内膜面积/中膜面积比值。IP3-type1和type2基因敲除小鼠的最大比值显著小于对照组。IP-3型2和3型基因敲除小鼠的平均比例显著低于对照组。在本研究中，IP3-R基因敲除小鼠颈动脉结扎模型中的内膜增生受到抑制。