Establishment of experimentally reflux esophagitis after distal partial gastrectomy in rats and pathophysiological investigation.

大鼠远端部分胃切除术后反流性食管炎的实验建立及病理生理学研究。

• 批准号: 14571233
• 负责人: OMURA Nobuo
• 金额: $ 1.54万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571233/
• 关键词: reflux esophagitis; distal partial gastrectomy; duodenal juice; rat; bile; pancreatic juice; 胃切除後逆流性食道炎

【Background】 Duodenal juice and acid in the remnant stomach would play important roll in reflux esophagitis after distal partial gastrectomy. We could make experimentally reflux esophagits in rats with anterectomy and gastroduododenal anastomosis (Biliroth I method). However, occurrence ratio of esophagitis and survival ratio were low (report in 2002). We retried to make some model for reflux esophagitis after gastrectomy. 【Materials and method】 We used five group of rats. Group 1 ; the control group, Group 2 ; gastroduodenal anastomosis after antrectomy, Group 3 ; covered with a thin catheter around duodenum at distal side of papilla vater in group 2, Group 4 ; ligation of limiting ridge (between the glandular portion and the forestomcb) in group 2, Group 5 ; ligation of limiting ridge in group 3. The animals were sacrifled 2 weeks after the start of experiment. We investigated the lesion of the esophagus macroscopically and histologically and measured remnant gastric pH. 【Results】 (1) Survival ratio : 100% in group 1, 50% in group 2, 50% in group 3, 40% in group 4, 40% in group 5, respectively. (2) Occurrence ratio of esophagitis 0% in group 1, 40% in group 2, 60% in group 3, 75% in group 4, 100% in group 5. (3) Histological examination : Marked thickening of the esophageal epitheliuxn, the elongation of papillae of the lainina propria into the epithelium and infiltration of inflammatory cells in the lamina propria were remarkable in the rats with esophagitis. 【Conclusion】 Ligation of limiting ridge was an important point in experimentally reflux esophagitis after distal gastrectomy.

[背景]十二指肠液和残胃酸在远端胃部分切除术后反流性食管炎中起重要作用。我们可以用前切除和胃十二指肠吻合术(Biliroth I法)制作实验性的大鼠反流食道。然而，食管炎的发生率和存活率都很低(2002年报道)。我们尝试制作胃切除术后反流性食管炎的动物模型。[材料与方法]选用5组大鼠。第1组为对照组，第2组为对照组，第3组为胃十二指肠切除后吻合组，第2组为第4组，在十二指肠乳头Vater远端用细导管覆盖，第2组为第5组，结扎限脊(腺体与前孔之间)，第3组为结扎限脊，实验开始2周后剖腹剖腹。我们对食道病变进行了大体观察和组织学检查，并测量了残胃的pH值。[结果](1)存活率：第1组为100%，第2组为50%，第3组为50%，第4组为40%，第5组为40%。(2)食管炎发生率：1组为0%，2组为40%，3组为60%，4组为75%，5组为100%。(3)组织学检查：食管炎大鼠食道上皮明显增厚，固有层乳头伸长，固有层炎性细胞浸润。[结论]结扎限脊是远端胃切除后实验性反流性食管炎的重要环节。