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Development of pancreatic stem cell and β-cell for cell therapy

开发用于细胞治疗的胰腺干细胞和β细胞

基本信息

批准号：
14571236
负责人：
TSUCHIYA Mariko
金额：
$ 2.18万
依托单位：
Tokyo Women's Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Maf is a family of transcription factor proteins, and maf A is a strong transactivator of insulin in cell lines. The induction of large mafs expression in rat pancreas under acute ischemic conditions in our previous study suggests that mafs may be involved not only in the regulation of cell function, but also in the development and differentiation of the pancreas. The present study investigated the expression profiles of the large maf family in the porcine pancreas and in primary culture cells. In the newborn pancreas tissue, the formation of islets was not clear compared with that in the adult pancreas tissue. Maf A- and c-positive cells were more diffuse and intensely stained in the pancreas tissue scatted throughout with fewer maf B positive clustering cells around at centroacinar area. In contrast, islet formation was more apparent and positive staining for maf A and c tended to be prominent in the islets of the adult pancreas tissue, As to endocrine function, maf A corresponds to … More insulin-and c-maf to glucagon-secreting cells, based on the results of a double staining study. Mafs mRNA levels measured by real-time PCR were overall expressed in the pancreas tissue. Mafs mRNA expression was also detectable in the primary cultures of endocrine cells freshly isolated from the newborn pancreas tissue. Each maf may be regulatory expressed and specific and independent transactivator of endocrine function. Maf B may also have other potentials for structure formation or hormone secretion. Fetal and adult human pancreas tissues stained positive for maf similarly as porcine, suggesting that porcine pancreas can be one prototype of human pancreas. Preliminarily, pancreas was small size but formed in newborn of c-maf knockout mice. In conclusion, large mafs were identified in pancreas and cultured endocrine cells, and the expression level and localization differ in newborn and adlut pancreas tissue. Mafs may play roles in establishing endocrine function during pancreatic endocrine cell differentiation in independent and specific manner. Less

Maf是一个转录因子蛋白家族，maf A是细胞系中胰岛素的强反式激活因子。我们先前的研究发现，急性缺血条件下，大鼠胰腺中mafs的大量表达被诱导，这表明mafs不仅参与细胞功能的调节，而且还参与胰腺的发育和分化。本研究调查了maf大家族在猪胰腺和原代培养细胞中的表达谱。与成人胰腺组织相比，新生胰腺组织中胰岛的形成不明显。Maf A和c阳性细胞在胰腺组织中分布较广，染色较强，散在分布，在腺泡中心区maf B阳性细胞较少。与此相反，成人胰腺组织的胰岛形成更明显，maf A和c的阳性染色倾向于突出。 ...更多信息胰岛素和c-MAF对胰高血糖素分泌细胞的作用。通过实时PCR测量的Mafs mRNA水平在胰腺组织中整体表达。Mafs mRNA的表达也可检测到的新生胰腺组织新鲜分离的内分泌细胞的原代培养物。每个maf都是调节性表达的，是内分泌功能的特异性和独立的反式激活因子。Maf B也可能具有其他结构形成或激素分泌的潜力。胎儿和成人胰腺组织与猪胰腺组织一样呈maf阳性，提示猪胰腺可能是人胰腺的原型之一。结果表明，c-maf基因敲除小鼠的胰腺体积较小，但在新生儿中形成。总之，在胰腺和培养的内分泌细胞中发现了大的mafs，并且在新生和成人胰腺组织中的表达水平和定位不同。Mafs可能在胰腺内分泌细胞分化过程中以独立和特异的方式参与内分泌功能的建立。少