Development of improving method for respiratory function in newborn diaphragma hernia

新生儿膈疝呼吸功能改善方法的研制

• 批准号: 14571255
• 负责人: TAKAHASHI Nobumasa
• 金额: $ 2.56万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571255/
• 关键词: myocardial regeneration; monocyte; tissue regeneration; 末梢血; 幹細胞; CD34抗体; 血管内皮前駆細胞; カテーテル

Although it has been reported that bone marrow cell could be introduced to cardiomyocyte by the stimulation of 5-azacytidine, its efficacy has been low and many points must be resolved were remained. We studied the new method for myocardial regeneration using peripheral monocyte.Method : Peripheral monocyte were collected by using monocyte fraction collector from peripheral blood. Total ammount of monocyte derived from this method was 1.8x10^9 cells. CD14 positive cells count was 0.06x10^7. Moncyte rich fraction was injected to the lower limb of painful ishemic 7 patients suffering ASO.Results : API was improved from 0.32 to 0.49 after injection of monocyte rich fraction. The need of use of anti-pain drug were dramacally reduced in all patients. 4 patients stopped the use of pentazocin. We consider that this effect is produced by the neovascularization by monocytes in the ischemic limb. This mechanism of neovascularization will be the new method for myocardial regeneration in the oschemin myocardium after infarction. We think that the method for getting monocyte fraction is very easy, so that we can use this method in the operating room for cardiac surgery.

尽管有报道称通过5-氮杂胞苷的刺激可以将骨髓细胞引入心肌细胞，但其功效较低，仍有许多问题需要解决。我们研究了利用外周单核细胞进行心肌再生的新方法。方法：采用单核细胞组分收集器从外周血中收集外周单核细胞。由该方法获得的单核细胞总量为1.8x10^9个细胞。 CD14 阳性细胞计数为 0.06x10^7。将富含 Moncyte 的组分注射到 7 名患有 ASO 的疼痛性缺血患者的下肢。结果：注射富含单核细胞的组分后，API 从 0.32 改善到 0.49。所有患者使用抗痛药的需求均显着减少。 4名患者停止使用喷他佐星。我们认为这种效应是由缺血肢体中的单核细胞新生血管产生的。这种新生血管机制将成为梗塞后oschemin心肌再生的新方法。我们认为获得单核细胞分数的方法非常简单，因此我们可以在手术室进行心脏手术时使用该方法。