The efficacy of partial liquid ventilation on lung protection during hypotension and cardiac arrest

部分液体通气对低血压和心脏骤停期间肺保护的效果

• 批准号: 14571257
• 负责人: SHINOZUKA Norihiro
• 金额: $ 2.18万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571257/
• 关键词: lung procurement; lung transplant after cardiac arrest; partial liquid ventilation; lung compliance; airway resistance; lung elastance; 死体肺移植; コンプライアンス; IL-8

Rationale : We previously reported that partial liquid ventilation (PLV) suppressed lung injury during cardiac arrest in the experimental rabbit model. We next investigated the efficacy of PLV in donor lung protection in lung transplantation from non-heart beating donors.Methods : We utilized eleven sets of female beagle dogs and divided into two groups : (a) conventional ventilation (GV, n=6), (b) partial liquid ventilation (PLV, n=5). While one-hour hypotension less than 50mmHg followed by 2-hour cardiac arrest was maintained, conventional ventilation or PLV had been performed for ventilation. Left donor lung retrieval and left single lung transplantation under conventional ventilation were performed. During the study period, arterial blood gas analysis, pulmonary resistance and elastance, and tissue Interleukin-8 (IL-8) concentration were serially measured. Donor lung histology was observed and assessed with histological evaluation scores. In 3 cases (GV:n=1, PLV:n=2) pulmonary resi … More stance and elastacne were measured even after the transplantation until the end of experiment.Results : There was no significant difference in arterial oxygen tension between the two groups during the study period. Histological evaluation score in both groups elevated gradually after transplantation, and the GV group showed its score at 6 hours after transplantation significantly higher than the PLV group. Pulmonary resistance and elastance gradually increased during the study period until lung retrieval, and there was no significant difference between the GV group and PLV group. IL-8 maintained its value as high as baseline level during the study period in both groups and there was no statistical difference between the two groups. According to the results of elastacne, resistance and histological evaluation score after the transplantation, there was correlation between histological scores and elastance or resistance.Conclusion : The histological analysis suggested PLV suppresses lung injury. There was correlation between the histological score and elastacne or resistance. And there is a possibility that the measurement of elastance or resistance might be one of the methods for the evaluation of lung injury. Less

理论基础：我们先前报道部分液体通气法(PLV)可抑制实验性兔心脏骤停时的肺损伤。方法：选用11只雌性比格犬，随机分为两组：(A)常规通气组(GV，n=6)；(B)部分液体通气组(PLV，n=5)。在维持低血压低于50毫米汞柱的1小时后心脏骤停2小时的同时，采用常规机械通气或PLV进行机械通气。在常规机械通气下行左供肺取材和左单肺移植。测定动脉血气分析、肺阻力和肺弹性、肺组织白介素8(IL-8)浓度。观察供体肺组织学变化，并用组织学评分进行评价。肺移植…3例(GV：N=1，PLV：N=2)结果：两组患者的动脉血氧分压在研究期间无明显差异。两组移植后组织学评分均逐渐升高，GV组移植后6h评分明显高于PLV组。肺阻力和肺弹性在研究期间逐渐增加，直到肺恢复，GV组和PLV组之间无显著差异。两组IL-8在研究期间均维持在基线水平，两组间差异无统计学意义。根据移植后弹力、抵抗力和组织学评分结果，组织学评分与弹性或抵抗力之间存在相关性。结论：PLV对肺损伤有抑制作用。组织学评分与弹性粉刺或抵抗力有关。弹性或阻力的测定有可能成为评价肺损伤的方法之一。较少

项目成果

The Efficacy of Partial Liquid Ventilation on Lung Protection during Hypotension and Cardiac Arrest

部分液体通气对低血压和心脏骤停期间肺保护的功效

• 发表时间: 2005
• 期刊: Journal of Heart and Lung Transplantation 24・6
• 作者: Yoshida S;Sekine Y;et al.
• 通讯作者: et al.