Quantitative analysis of high pressure ventilation stress on lung cell with one lung ventilation

单肺通气高压通气应激对肺细胞的定量分析

• 批准号: 13671563
• 负责人: SHINOZUKA Norihiro
• 金额: $ 2.3万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671563/
• 关键词: c-fos; RT-PCR; one lung ventilation; 人工呼吸

We reported that we could confirm c-fos gene expression in a lung tissue under high positive end-expiratory pressure ventilation. This gene expression means that a lung cell is suffered with share stress. We confirm that 30 minutes high PEEP ventilation is enough for c-fos gene expression and quantitative analysis of c-fos gene expression detects difference of degree of lung surface share stress.We applied one lung ventilated rat to detect c-fos mRNA expression induced by high PEEP ventilation. Our result suggest ventilation with high PEEP may induce c-fos mRNA expression more than low PEEP ventilation and there is different tendency between ventilated and non-ventilated rat lung cell gene expressions. So we tried to use both side lung lobes, ventilated and non-ventilated, for analysis of difference of gene expression. Blood flow should be affected by gravity and ventilation may induce share stress in upper area. Abdominal side lung cell expressed more than backside lung cell. Out result suggest the tendency of higher PEEP induces more stress gene expression but some extent difference between rats and the reasons are surgical procedure and total lung maneuver so we applied no total lung maneuver.Some rats revealed ventilated lung cell expressed c-fos mRNA more than non-ventilated side lung cell and abdominal side also showed more gene expression. These results support our hypothesis that higher pressure ventilation suffered more stress and induce more stress gene expression. But some rats show inverse expression against our hypothesis and no expression even though in ventilated side lung cell.Now we scheduled Nothern-Plot analysis for help to reduce artificial noise, such as surgical procedure, and feedback to RT-PCR result of c-fos gene expression.

我们报告，我们可以确认c-fos基因表达在肺组织在高呼气末正压通气。这种基因表达意味着肺细胞承受着压力。本研究证实高PEEP通气30分钟对c-fos基因表达是足够的，定量分析c-fos基因表达可检测肺表面分担应激程度的差异。我们的结果表明，高PEEP通气比低PEEP通气更能诱导c-fos mRNA的表达，并且通气和非通气大鼠肺细胞基因表达存在不同的趋势。因此，我们尝试使用通气和非通气的两侧肺叶来分析基因表达的差异。血液流动应受重力和通风的影响，可能会导致共享应力在上部区域。腹侧肺细胞表达高于背侧肺细胞。本研究结果提示，高PEEP诱导更多的应激基因表达，但大鼠之间存在一定程度的差异，原因是手术操作和全肺操作，因此我们不进行全肺操作，部分大鼠通气侧肺细胞c-fos mRNA表达高于非通气侧肺细胞，腹侧也有更多的基因表达。这些结果支持了我们的假设，即更高的压力通气遭受更多的压力，并诱导更多的应激基因表达。但也有部分大鼠的c-fos基因表达与我们的假设相反，即使在通气侧肺细胞中也没有表达，因此我们安排了Nothern-Plot分析，以帮助减少人为的噪音，如手术操作，并反馈到RT-PCR结果的c-fos基因表达。