权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Management of artificial respiration worsens acute lung injury.-Investigation and influence of mechanism by molecular biological approach-

人工呼吸的处理使急性肺损伤恶化。-通过分子生物学方法研究其机制及其影响-

基本信息

批准号：
14571434
负责人：
MORIKAWA Osamu
金额：
$ 1.73万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571434/
关键词：
alveolar type II cell cell stretch

项目摘要

Alveolar epithelial cells type II (AEC-II) are thought to play an important role in the pathogenesis of acute lung injury because AEC-II can produce many types of pro-inflammatory cytokines and chemokines. Especially, Interleukin-8 (IL-8) plays an important role in this inflammatory response. AEC-II are exposeed to mechanical stretch during breathing and mechanical ventilation. Increased stretch may contribute to lung injury. The purpose of this study is to elucidate the effects of cell stretch on endotoxin-induced interleukin-8 (IL-8) production from cultured rat AEC-II.After isolation of rat AEC-II, cells were cultured for 48h. LPS (lipopolysaccaraide) increased rat GRO/CINC (IL-8) measured by ELISA in rat alveolar type II cell cultured media. Cells were stretched using mechanical stretch machine (Flexcell). At 3h, 6h, 12h and 24h, the cell media were collected and GRO/CINC (IL-8) were measured by ELISA. The concentration of GRO/CINC (IL-8) increased time-dependently in 24h. LPS & cell Stretch induced additional increase compared with each study.These results showed cell damage induced by cell stretch was similar to ventilator-induced lung injury and LPS & stretch means mechanical ventilation in septic state in clinical. This model may help for future therapy and mechanizm for acute lung injury induced by sepsis.

II型肺泡上皮细胞（AEC-II）被认为在急性肺损伤的发病机制中发挥重要作用，因为AEC-II可以产生多种促炎细胞因子和趋化因子。尤其是白介素 8 (IL-8) 在这种炎症反应中发挥着重要作用。 AEC-II 在呼吸和机械通气期间受到机械拉伸。增加拉伸可能会导致肺损伤。本研究的目的是阐明细胞拉伸对培养的大鼠 AEC-II 内毒素诱导的白细胞介素 8 (IL-8) 产生的影响。分离大鼠 AEC-II 后，将细胞培养 48 小时。通过 ELISA 在大鼠肺泡 II 型细胞培养基中测量，LPS（脂多糖）增加了大鼠 GRO/CINC (IL-8)。使用机械拉伸机（Flexcell）拉伸细胞。在3小时、6小时、12小时和24小时时，收集细胞培养基并通过ELISA测量GRO/CINC(IL-8)。 GRO/CINC (IL-8) 浓度在 24 小时内呈时间依赖性增加。与每项研究相比，LPS和细胞拉伸引起额外的增加。这些结果表明，细胞拉伸引起的细胞损伤与呼吸机引起的肺损伤相似，LPS和拉伸意味着临床上脓毒症状态下的机械通气。该模型可能有助于脓毒症引起的急性肺损伤的未来治疗和机制。