An investigation of mechanisms of the tissue pressure-mediatedtransfection method using microdevices for cell stretch

点击翻译按钮获取中文摘要

We have developed tissue pressure-mediated in vivo transfection method for delivering naked nucleic acids. In the present study, we aimed to clarify the mechanism of the tissue pressure-mediated transfection method by in vitro experiments. Considering the process of the tissue pressure-mediated transfection method, cells in the pressurized organs/tissues should be stretched and the stretch stimuli may play an important role. Thus, we developed microdevices for cell stretch, and investigated the effects of stretch stimuli on the permeability of the endothelial cell monolayer constructed on the device and on the transfection efficiency of naked plasmid DNAs to the cells cultured on the device. As a results, it was suggested that the stretch stimuli increased the permeability of the endothelial cells transiently. Therefore, the mechanism of the tissue pressure-mediated transfection method was clarified partly.